Flow Cytometry of human PBMCs using CD86 Monoclonal Antibody (BU63).

Anti-CD86 Antibody Clone BU63

$ 298.00
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SKU: 942-MSM1
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
942-MSM1-CS1 Size: 0.5 ml, Tag: Unconjugated
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SKU: 942-MSM1-CS1
CD86 General Information
Alternate Names
Cluster of Differentiation 86, CD86, B7-2, B7.2
Molecular Weight
70kDa
Chromosomal Location
3q13.33
Curated Database and Bioinformatic Data
Gene SymbolCD86
Entrez Gene ID942
Ensemble Gene IDENSG00000114013
RefSeq Protein Accession(s)NP_001193853, NP_001193854, NP_795711, NP_008820, NP_787058
RefSeq mRNA Accession(s)NM_176892, NM_001206924 NM_006889, NM_175862, NM_001206925
RefSeq Genomic Accession(s)NG_029928, NC_018914, NC_000003
UniProt ID(s)P42081, A0A0X9R4E0, A8K632
UniGene ID(s)P42081, A0A0X9R4E0, A8K632
HGNC ID(s)1705
Cosmic ID(s)CD86
KEGG Gene ID(s)hsa:942
PharmGKB ID(s)PA26243
General Description of CD86.
Recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein, a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes, dendritic cells, at very low density on resting B, T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28, CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin, Reed Sternberg (HRS) cells in Hodgkin's disease.

Human, Mouse, and Rat Anti-CD86 Antibody Product Attributes

Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-CD86 Antibody Clone: BU63
Clone BU63 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse, and Rat CD86 Positive Control Sample: 293T or Jukat cells. Monocytes, dendritic cells, activated T, B, natural killer cells in lymph node or tonsil.
Cellular Localization of Antibody Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: ARH-77 (B-lymphoblastoid cell line)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD86 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD86 expression as measured by anti-CD86 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ ++ + ++ ++ + + ++ ++ ++ ++ + ++ + - + ++ ++ ++ ++
CD86 Variability ++ ++ ++ + ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + + ++

Limitations and Warranty

enQuire Bio's CD86 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD86 Antibody Clone BU63