enQuire-Bio-QAB94-PE-100Tests-anti-CD86-antibody-10

Anti-CD86 Antibody [IT2.2]

$ 88.00$ 238.00
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SKU: QAB94
Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: PE
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB94-PE-25Tests
CD86 General Information
Alternate Names
B7-2, B7.2
Curated Database and Bioinformatic Data
Gene SymbolCD86
Entrez Gene ID942
Ensemble Gene IDENSG00000114013
RefSeq Protein Accession(s)NP_001193853, NP_001193854, NP_795711, NP_008820, NP_787058
RefSeq mRNA Accession(s)NM_176892, NM_001206924 NM_006889, NM_175862, NM_001206925
RefSeq Genomic Accession(s)NG_029928, NC_018914, NC_000003
UniProt ID(s)P42081, A0A0X9R4E0, A8K632
UniGene ID(s)P42081, A0A0X9R4E0, A8K632
HGNC ID(s)1705
Cosmic ID(s)CD86
KEGG Gene ID(s)hsa:942
PharmGKB ID(s)PA26243
General Description of CD86.
The IT2.2 antibody reacts with human CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 can also bind a second B7 ligand known as CD80 (B7-1). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.The IT2.2 antibody may be used as a marker for CD86 expression on B cells, macrophages, and dendritic cells. It is reported to be cross-reactive with Rhesus, Cynomolgus and Common marmoset CD86.

Human Anti-CD86 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD86 Antibody Clone: IT2.2
Clone IT2.2 Host and Isotype: Mouse IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD86 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD86 expression as measured by anti-CD86 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ ++ + ++ ++ + + ++ ++ ++ ++ + ++ + - + ++ ++ ++ ++
CD86 Variability ++ ++ ++ + ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + + ++

Selected References

Morgado P, Ong Y-C, Boothroyd JC, and Lodoen MB. 2011. Infect. Immun. 79: 4401-4412. (in vitro stimulation)Kap YS, van Meurs M, van Driel N, Koopman G, Melief M-J, Brok HPM, Laman JD, and ‘t Hart BA. 2009. J. Histochemistry & Cytochemistry. 57: 1159-1167. (Immunohistochemistry - frozen tissue: Rhesus, Cynomolgus, Common marmoset)Teleshova N, Kenney J, Willliams V, Van Nest G, Marshall J, Lifson LM, Sivin I, Dufour J, Bohm R, Gettie A, and Pope M. 2006. J. Leukoc. Ciol. 79:257-267. (Flow cytometry - rhesus macaque)Conti L, Casetti R, Cardone M, Varano B, Martino A, Belardelli F, Poccia F, and Gessani S. 2005. J. Immunol. 174: 252-260. (Flow cytometry, in vitro blocking)Esser MT, Graham DR, Coren LV, Trubey CM, Bess JW, Arthur LO, Ott DE, and Lifson JD. 2001. J. Virol. 75(13)6173-6182. (Western Blot)

Limitations and Warranty

enQuire Bio's Human Anti-CD86 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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