Human Anti-CD86 Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD86 Antibody Clone: IT2.2
Clone IT2.2 Host and Isotype: Mouse IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD86 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of CD86 expression as measured by anti-CD86 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | ++ | ++ | + | ++ | ++ | + | + | ++ | ++ | ++ | ++ | + | ++ | + | - | + | ++ | ++ | ++ | ++ |
CD86 Variability | ++ | ++ | ++ | + | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | + | + | ++ |
Selected References
Morgado P, Ong Y-C, Boothroyd JC, and Lodoen MB. 2011. Infect. Immun. 79: 4401-4412. (in vitro stimulation)Kap YS, van Meurs M, van Driel N, Koopman G, Melief M-J, Brok HPM, Laman JD, and Ât Hart BA. 2009. J. Histochemistry & Cytochemistry. 57: 1159-1167. (Immunohistochemistry - frozen tissue: Rhesus, Cynomolgus, Common marmoset)Teleshova N, Kenney J, Willliams V, Van Nest G, Marshall J, Lifson LM, Sivin I, Dufour J, Bohm R, Gettie A, and Pope M. 2006. J. Leukoc. Ciol. 79:257-267. (Flow cytometry - rhesus macaque)Conti L, Casetti R, Cardone M, Varano B, Martino A, Belardelli F, Poccia F, and Gessani S. 2005. J. Immunol. 174: 252-260. (Flow cytometry, in vitro blocking)Esser MT, Graham DR, Coren LV, Trubey CM, Bess JW, Arthur LO, Ott DE, and Lifson JD. 2001. J. Virol. 75(13)6173-6182. (Western Blot)
Limitations and Warranty
enQuire Bio's Human Anti-CD86 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
There are no reviews yet.