Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with CD99 Monoclonal Antibody (12E7).

Anti-CD99 / MIC2 Antibody Clone 12E7.

$ 298.00
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SKU: 4267-MSM2
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
4267-MSM2-CS1 Size: 0.5 ml, Tag: Unconjugated
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SKU: 4267-MSM2-CS1
CD99 / MIC2 General Information
Alternate Names
CD99 antigen, Cluster of differentiation 99, MIC2, single-chain type-1 glycoprotein
Molecular Weight
27-32kDa
Chromosomal Location
Xp22.33
Curated Database and Bioinformatic Data
Gene SymbolCD99
Entrez Gene ID4267
Ensemble Gene IDENSG00000002586
RefSeq Protein Accession(s)NP_002405, NP_001308298, NP_001116370, NP_001308296, NP_001308297, NP_001308299
RefSeq mRNA Accession(s)NM_001321368, NM_001321369, NM_001321370, NM_001122898, NM_002414, NM_001277710, NM_001321367, NR_135623
RefSeq Genomic Accession(s)NC_000023, NC_000024, NG_009174, NC_018934
UniProt ID(s)A0A096LP69, P14209
UniGene ID(s)A0A096LP69, P14209
HGNC ID(s)7082
Cosmic ID(s)CD99
KEGG Gene ID(s)hsa:4267
PharmGKB ID(s)PA30804
General Description of CD99 / MIC2.
Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X, Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript, are identified as bands of 30, 32kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor, MHS molecules, apoptosis of immature thymocytes, leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes,, granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis,, some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells, primitive peripheral neuroectodermal tumors.

Human Anti-CD99 / MIC2 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-CD99 / MIC2 Antibody Clone: 12E7.
Clone 12E7. Host and Isotype: Mouse IgG1 kappa
Anti-Human CD99 / MIC2 Positive Control Sample: MOLT-4 cells. Pancreas or Ewing s sarcoma
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: Tissue culture supernatant with 0.05% Azide.
Antibody Concentration: Not applicable
Antibody Purification Method:Culture Supernatant
Immunogen: Human acute lymphocytic leukemia T-cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD99 / MIC2 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CD99 antibody staining is expected to be primarily localized to the golgi apparatus.

Observed Antibody Staining Data By Tissue Type:

Variations in CD99 / MIC2 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the endometrial stroma in endometrium, cells in the red pulp in spleen, cells in the seminiferous ducts in testis, glandular cells in the cervix, uterine and prostate, hematopoietic cells in the bone marrow, islets of Langerhans in pancreas, ovarian stroma cells in the ovary, respiratory epithelial cells in the nasopharynx and squamous epithelial cells in the esophagus. More moderate antibody staining intensity was present in cells in the endometrial stroma in endometrium, cells in the red pulp in spleen, cells in the seminiferous ducts in testis, glandular cells in the cervix, uterine and prostate, hematopoietic cells in the bone marrow, islets of Langerhans in pancreas, ovarian stroma cells in the ovary, respiratory epithelial cells in the nasopharynx and squamous epithelial cells in the esophagus. Low, but measureable presence of CD99 / MIC2 could be seen inadipocytes in mesenchymal tissue, cells in the white pulp in spleen, endothelial cells in the colon, follicle cells in the ovary, glandular cells in the breast, duodenum, endometrium, epididymis and gallbladder, macrophages in lung, non-germinal center cells in the tonsil and squamous epithelial cells in the tonsil. We were unable to detect CD99 / MIC2 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD99 / MIC2 expression as measured by anti-CD99 / MIC2 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - ++ - + - +++ ++ - - + - - - - - - - - - -
CD99 Variability ++ ++ + ++ ++ ++ ++ ++ + ++ ++ + ++ ++ ++ ++ ++ + ++ ++

Limitations and Warranty

enQuire Bio's CD99 / MIC2 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD99 / MIC2 Antibody Clone 12E7.