Formalin-fixed, paraffin-embedded human Ewing's sarcoma stained with CD99 Monoclonal Antibody (SPM586).
Formalin-fixed, paraffin-embedded human Ewing's sarcoma stained with CD99 Monoclonal Antibody (SPM586).

Anti-CD99 / MIC2 Antibody Clone SPM586

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 4267-MSM1X
Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgM kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
4267-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: 4267-MSM1X-P1
CD99 / MIC2 General Information
Alternate Names
CD99 antigen, Cluster of differentiation 99, MIC2, single-chain type-1 glycoprotein
Molecular Weight
27-32kDa
Chromosomal Location
Xp22.33
Curated Database and Bioinformatic Data
Gene SymbolCD99
Entrez Gene ID4267
Ensemble Gene IDENSG00000002586
RefSeq Protein Accession(s)NP_002405, NP_001308298, NP_001116370, NP_001308296, NP_001308297, NP_001308299
RefSeq mRNA Accession(s)NM_001321368, NM_001321369, NM_001321370, NM_001122898, NM_002414, NM_001277710, NM_001321367, NR_135623
RefSeq Genomic Accession(s)NC_000023, NC_000024, NG_009174, NC_018934
UniProt ID(s)A0A096LP69, P14209
UniGene ID(s)A0A096LP69, P14209
HGNC ID(s)7082
Cosmic ID(s)CD99
KEGG Gene ID(s)hsa:4267
PharmGKB ID(s)PA30804
General Description of CD99 / MIC2.
Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. This antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes,, granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis,, some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells, primitive peripheral neuroectodermal tumors. This MAb shows a very similar reactivity to other CD99 MAbs (e.g. O13, 12E7, or HBA-71), is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

Human and Rat Anti-CD99 / MIC2 Antibody Product Attributes

Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (5-10ul/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-CD99 / MIC2 Antibody Clone: SPM586
Clone SPM586 Host and Isotype: Mouse IgM kappa
Anti-Human and Rat CD99 / MIC2 Positive Control Sample: Pancreas or Ewing s sarcoma
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: ImmunogenImmunogenetics, 1992, 35(4):283-5

Storage, Stability

Store at 2 to 8° C (refrigerate).. Stable for 24 months when properly stored.

Limitations

This antibody is available for research use only, is not approved for use in diagnostics.

Warranty

This antibody is good to the last drop. If you have any problems, call or email us immediately.

Stocked, validated by enQuire Bio, manufactured using only the highest quality standards by NeoBiotechnologies.

Search terms: 12E7; E2 antigen; MIC 2X; MIC 2Y; MIC2; Protein MIC2; Surface antigen MIC2; T-cell surface glycoprotein E2; anti-12E7 antibody; anti-E2 antigen antibody; anti-MIC 2X antibody; anti-MIC 2Y antibody; anti-MIC2 antibody; anti-Protein MIC2 antibody; anti-Surface antigen MIC2 antibody; anti-T-cell surface glycoprotein E2 antibody
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD99 / MIC2 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CD99 antibody staining is expected to be primarily localized to the golgi apparatus.

Observed Antibody Staining Data By Tissue Type:

Variations in CD99 / MIC2 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the endometrial stroma in endometrium, cells in the red pulp in spleen, cells in the seminiferous ducts in testis, glandular cells in the cervix, uterine and prostate, hematopoietic cells in the bone marrow, islets of Langerhans in pancreas, ovarian stroma cells in the ovary, respiratory epithelial cells in the nasopharynx and squamous epithelial cells in the esophagus. More moderate antibody staining intensity was present in cells in the endometrial stroma in endometrium, cells in the red pulp in spleen, cells in the seminiferous ducts in testis, glandular cells in the cervix, uterine and prostate, hematopoietic cells in the bone marrow, islets of Langerhans in pancreas, ovarian stroma cells in the ovary, respiratory epithelial cells in the nasopharynx and squamous epithelial cells in the esophagus. Low, but measureable presence of CD99 / MIC2 could be seen inadipocytes in mesenchymal tissue, cells in the white pulp in spleen, endothelial cells in the colon, follicle cells in the ovary, glandular cells in the breast, duodenum, endometrium, epididymis and gallbladder, macrophages in lung, non-germinal center cells in the tonsil and squamous epithelial cells in the tonsil. We were unable to detect CD99 / MIC2 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD99 / MIC2 expression as measured by anti-CD99 / MIC2 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - ++ - + - +++ ++ - - + - - - - - - - - - -
CD99 Variability ++ ++ + ++ ++ ++ ++ ++ + ++ ++ + ++ ++ ++ ++ ++ + ++ ++

Limitations and Warranty

enQuire Bio's CD99 / MIC2 Anti-Human, Rat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “Anti-CD99 / MIC2 Antibody Clone SPM586”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   1825 Lawrence St, Ste 115   Denver, CO 80202
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

Anti-CD99 / MIC2 Antibody Clone SPM586

https://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-4267-MSM1X-P1-anti-CD99-MIC2-antibody-300x225.jpeg