Formalin-fixed, paraffin-embedded human Tonsil stained with CDC2 Monoclonal Antibody (CDC2/112)
Formalin-fixed, paraffin-embedded human Tonsil stained with CDC2 Monoclonal Antibody (CDC2/112)

Anti-Cdc20 Antibody Clone CDC20/1102

$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 991-MSM2
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
991-MSM2-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
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SKU: 991-MSM2-P1
Cdc20 General Information
Alternate Names
Cell-Division Cycle Protein 20, CDC20
Molecular Weight
55kDa
Chromosomal Location
1p34.2
Curated Database and Bioinformatic Data
Gene SymbolCDC20
Entrez Gene ID991
Ensemble Gene IDENSG00000117399
RefSeq Protein Accession(s)NP_001246
RefSeq mRNA Accession(s)NM_001255
RefSeq Genomic Accession(s)NC_000001, NC_018912
UniProt ID(s)Q12834
UniGene ID(s)Q12834
HGNC ID(s)1723
Cosmic ID(s)CDC20
KEGG Gene ID(s)hsa:991
PharmGKB ID(s)PA26257
General Description of Cdc20.
Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro, exists in a complex with both cyclin B, a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF), the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20, Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.

Human Anti-Cdc20 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (2-4ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Cdc20 Antibody Clone: CDC20/1102
Clone CDC20/1102 Host and Isotype: Mouse IgG1 kappa
Anti-Human Cdc20 Positive Control Sample: Ramos or HeLa cells. Tonsil or gastric carcinoma.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human Cdc20 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Cdc20 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CDC20 antibody staining is expected to be primarily localized to the cytosol and nucleoplasm. There is variability in either the signal strength or the localization of signal in cytosol and nucleoplasm from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in Cdc20 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the appendix, small intestine, stomach. More moderate antibody staining intensity was present in glandular cells in the appendix, small intestine, stomach. Low, but measureable presence of Cdc20 could be seen in cells in the tubules in kidney, glandular cells in the breast and salivary gland, non-germinal center cells in the lymph node, respiratory epithelial cells in the nasopharynx and urothelial cells in the urinary bladder. We were unable to detect Cdc20 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Cdc20 expression as measured by anti-Cdc20 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ - +++ ++ ++ ++ + + ++ ++ ++ ++ + ++ + ++ + ++ + ++
CDC20 Variability + ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ +++ ++ ++ + +++ ++ ++ ++

Limitations and Warranty

enQuire Bio's Cdc20 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Cdc20 Antibody Clone CDC20/1102

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