Discontinued
Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:10-1:25 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Clonality: Monoclonal
Anti-CDX2 Antibody Clone: AMT28
Host and Isotype: Mouse IgG1, kappa
Recommended Positive Control Sample: Colon carcinoma
Cellular Localization of Antibody AMT28 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Prokaryotic recombinant protein corresponding to the 180 amino acid N-terminal region of the human cdx2 molecule.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
CDX2 Information for Pathologists
Summary:
Homeobox gene that encodes a nuclear transcription factor critical for intestinal embryonic development; relatively specific for intestinal epithelium (but see positive staining below). Terminology Also called caudal-related homeobox gene 2, caudal type homeobox transcription factor 2. Homologue of Drosophila melanogaster homeobox gene-caudal. Uses by pathologistsCommon Uses By Pathologists:
Fairly specific marker of GI origin for adenocarcinomas, but also stains selected adenocarcinomas of other sites indicated below (Am J Surg Pathol 2003;27:303); can use to determine origin of metastatic adenocarcinoma as part of panel (Arch Pathol Lab Med 2007;131:1561). Distinguish primary and secondary colorectal adenocarcinomas (Arch Pathol Lab Med 2005;129:920). Distinguish primary bladder adenocarcinoma (CDX2-, villin-) from colorectal carcinoma extending / metastatic to bladder (CDX2+, villin+, Mod Pathol 2005;18:1217). Distinguish mucinous bronchioloalveolar adenocarcinoma (CDX2-) from metastatic mucinous colorectal adenocarcinoma (CDX2+, Am J Clin Pathol 2004;122:421). Microscopic (histologic) imagesLimitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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