Immunohistochemical staining of Chromogranin A  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Chromogranin A [SP12] Antibody (cGMP).

$ 128.00$ 438.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD102
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Rabbit Rabbit IgG

Product NumberDescriptionPrice
QIVD102-0.1ml Size: 0.1 ml, Format: Concentrate $ 128.00
QIVD102-0.5ml Size: 0.5 ml, Format: Concentrate $ 278.00
QIVD102-1ml Size: 1 ml, Format: Concentrate $ 438.00
QIVD102-6ml Size: 6 ml, Format: Predilute $ 178.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
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SKU: QIVD102-0.1ml
Chromogranin A General Information
Alternate Names
Molecular Weight
50.7 kDa
Chromosomal Location
[chr: CHR_HSCHR14_7_CTG1] [chr_start: 92923080] [chr_end: 92935293] [strand: 1]; q32.12 [chr: 14] [chr_start: 92923150] [chr_end: 92935285] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolCHGA
Entrez Gene ID1113
RefSeq Protein Accession(s)XP_011534672; NP_001266; NP_001288619
RefSeq mRNA Accession(s)XM_011536370; NM_001301690; NM_001275
RefSeq Genomic Accession(s)NC_000014; NT_187601
UniProt ID(s)P10645
PharmGKB ID(s)PA26461
KEGG Gene ID(s)hsa:1113
General Description of Chromogranin A .
This antibody recognizes Chromogranin A. Chromogranin A (a protein of 439 amino acid which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Chromogranin A Antibody Clone: SP12
Host and Isotype: Rabbit Rabbit IgG
Recommended Positive Control Sample: Pancreas
Cellular Localization of Antibody SP12 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Recombinant protein encoding human Chromogranin A.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Chromogranin A Information for Pathologists

Summary:

Granin protein located in secretory vesicles of neurons and endocrine cells (Clin Invest Med 1995;18:47, Endocr Rev 2011;32:755). Immunostain is specific but not sensitive for neuroendocrine cells; more sensitive in well differentiated vs. poorly differentiated tumors. Antibody binds acidic glycoproteins in the soluble fraction of neurosecretory granules. Serum levels may not be useful for diagnosis (Biomarkers 2012;17:186), but changes in levels may reflect response to therapy or recurrence (Folia Biol (Praha) 2011;57:173). Terminology

Common Uses By Pathologists:

Commonly used neuroendocrine marker (also synaptophysin and CD56) for normal cells and neuroendocrine tumors. Helps differentiate pheochromocytoma (almost always positive) from adrenocortical carcinoma (almost always negative, Am J Surg Pathol 2010;34:423). Microscopic (histologic) images Images hosted on PathOut server:.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Chromogranin A [SP12] Antibody (cGMP).