Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
|Recommended Dilution (Conc)||1:50-1:100|
|Pretreatment||Citrate Buffer pH 6.0|
|Incubation Parameters||30 min at Room Temperature|
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Anti-Chromogranin A Antibody Clone: EP38
Host and Isotype: Rabbit Rabbit IgG
Recommended Positive Control Sample: Pancreas Neuroendocrine Tumors
Cellular Localization of Antibody EP38 Staining: Cytoplasm
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide corresponding to residues near the C-terminus ofhuman Chromogranin A protein
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Chromogranin A Information for Pathologists
Summary:Granin protein located in secretory vesicles of neurons and endocrine cells (Clin Invest Med 1995;18:47, Endocr Rev 2011;32:755). Immunostain is specific but not sensitive for neuroendocrine cells; more sensitive in well differentiated vs. poorly differentiated tumors. Antibody binds acidic glycoproteins in the soluble fraction of neurosecretory granules. Serum levels may not be useful for diagnosis (Biomarkers 2012;17:186), but changes in levels may reflect response to therapy or recurrence (Folia Biol (Praha) 2011;57:173). Terminology
Common Uses By Pathologists:Commonly used neuroendocrine marker (also synaptophysin and CD56) for normal cells and neuroendocrine tumors. Helps differentiate pheochromocytoma (almost always positive) from adrenocortical carcinoma (almost always negative, Am J Surg Pathol 2010;34:423). Microscopic (histologic) images Images hosted on PathOut server:.
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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