Formalin-fixed, paraffin-embedded human Kidney Transplant stained with Complement 4d Monoclonal Antibody (SPM545).
Formalin-fixed, paraffin-embedded human Kidney Transplant stained with Complement 4d Monoclonal Antibody (SPM545).

Anti-Complement 4d Antibody Clone SPM545

$ 429.00
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SKU: 720-MSM1X
Species: Human
Tested Applications: ELISA, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
720-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 720-MSM1X-P1
Complement 4d General Information
Alternate Names
Complement C4-A, C4A, Often misspelled as Compliment C4
Molecular Weight
192kDa (predicted)
Chromosomal Location
6p21.3
Curated Database and Bioinformatic Data
Gene SymbolC4A
Entrez Gene ID720, 721
Ensemble Gene IDENSG00000227746, ENSG00000206340, ENSG00000244731, ENSG00000244207, ENSG00000224389, ENSG00000224639, ENSG00000228454, ENSG00000228267, ENSG00000236625
RefSeq Protein Accession(s)NP_001239133, NP_009224, XP_016885778, NP_001002029
RefSeq mRNA Accession(s)XM_017030289, NM_007293 NM_001252204NM_001002029
RefSeq Genomic Accession(s)NC_000006, NG_167248, NG_011638, NG_167247, NG_167245, NC_018917NG_011639, NC_018917, NG_167245, NG_113891, NG_167249, NC_000006
UniProt ID(s)Q13906, Q14835, Q9NPK5, Q9UIP5, P0C0L4, P01028, P78445, Q13160, Q14033, P0C0L4, P0C0L5
UniGene ID(s)Q13906, Q14835, Q9NPK5, Q9UIP5, P0C0L4, P01028, P78445, Q13160, Q14033, P0C0L4, P0C0L5
HGNC ID(s)1323, 1324
Cosmic ID(s)C4A, C4B
KEGG Gene ID(s)hsa:720, hsa:721
PharmGKB ID(s)PA25904, PA25903, PA25904, PA25903
General Description of Complement 4d.
This MAb is specific to Complement 4d (C4d), it reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation, degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces, extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas, lung allografts. As an established marker of antibody-mediated acute renal allograft rejection, its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection,, borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt, aggressive anti-rejection treatment.

Human Anti-Complement 4d Antibody Product Attributes

Species: Human
Tested Applications: ELISA, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: ELISA (For coating use Ab at 1-2ug of antibody/ml, order Ab without BSA), Immunofluorescence (1:50-1:100), Immunohistochemistry (IHC) (Formalin-fixed) (1:200-1:400 for 30 minutes at RT)
Clonality: Monoclonal
Anti-Complement 4d Antibody Clone: SPM545
Clone SPM545 Host and Isotype: Mouse IgG1 kappa
Anti-Human Complement 4d Positive Control Sample: Rejected Renal Transplant Tissue
Cellular Localization of Antibody Intracytoplasmic vacuoles of endothelial cells, Secreted
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human Complement 4d protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Complement 4d Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in Complement 4d antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of Complement 4d could be seen in. We were unable to detect Complement 4d in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's Complement 4d Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Complement 4d Antibody Clone SPM545

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