Analysis of Mass Spec data (dashed-line) of fractions stained with CPS1 / Carbamoyl-Phosphate Synthetase MS-QAVA™ monoclonal antibody [Clone: CPS1/1022] (solid-line), reveals that less than 0.5% of signal is attributable to non-specific binding of anti-CPS1 / Carbamoyl-Phosphate Synthetase [Clone CPS1/1022] to targets other than CPS1 protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in Jurkat, U202 and HeLa cells.
Analysis of Mass Spec data (dashed-line) of fractions stained with CPS1 / Carbamoyl-Phosphate Synthetase MS-QAVA™ monoclonal antibody [Clone: CPS1/1022] (solid-line), reveals that less than 0.5% of signal is attributable to non-specific binding of anti-CPS1 / Carbamoyl-Phosphate Synthetase [Clone CPS1/1022] to targets other than CPS1 protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in Jurkat, U202 and HeLa cells.

Anti-CPS1 / Carbamoyl-Phosphate Synthetase Antibody Clone CPS1/1022

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 1373-MSM1
Species: Human and Canine
Tested Applications: Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: MS-QAVA™ Validated

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Datasheets and Documentation
Product Datasheet
SKU: 1373-MSM1-P1
CPS1 / Carbamoyl-Phosphate Synthetase General Information
Alternate Names
Calcitonin, thyrocalcitonin, CALCA
Molecular Weight
~165kDa
Chromosomal Location
2q35
Curated Database and Bioinformatic Data
Gene SymbolCPS1
Entrez Gene ID1373
Ensemble Gene IDENSG00000021826
RefSeq Protein Accession(s)NP_001116106, XP_011508942, XP_011508944, NP_001866, XP_011508946, NP_001116105, XP_011508943, XP_011508945
RefSeq mRNA Accession(s)NM_001122633, XM_011510640, XM_011510642, NM_001122634, XM_011510644, NM_001875, XM_011510643
RefSeq Genomic Accession(s)NG_008285, NC_018913, NC_000002
UniProt ID(s)A0A024R454, P31327, B7ZAW0, Q6PEK7
UniGene ID(s)A0A024R454, P31327, B7ZAW0, Q6PEK7
HGNC ID(s)2323
Cosmic ID(s)CPS1
KEGG Gene ID(s)hsa:1373
PharmGKB ID(s)PA26840
General Description of CPS1 / Carbamoyl-Phosphate Synthetase.
This MAb recognizes a protein of 165kDa, identified as carbamoyl phosphate synthetase 1 (CPS1). This mitochondrial enzyme catalyzes synthesis of carbamoyl phosphate from ammonia, bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells.Deficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia. CPS1 is a hepatocyte specific protein that localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC s occur primarily in the stomach, but they are also found in many other organs. CPS1 may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of CPS1 correlates with smaller tumor size, longer patient survival. Occasionally, CPS1 is also found in gastric carcinomas as well as in a few other non-hepatic tumors.

Human Anti-CPS1 / Carbamoyl-Phosphate Synthetase Antibody Product Attributes

Species: Human.
Predicted Cross-Reactivity: Canine.
Tested Applications: Immunofluorescence, Immunohistochemistry (IHC). Additionally, this antibody has been validated by MS-QAVA™. Specificity has been quantitatively determined via Mass Spec.
Clonality: Monoclonal
Anti-CPS1 / Carbamoyl-Phosphate Synthetase Antibody Clone: CPS1/1022
Clone CPS1/1022 Host and Isotype: Mouse IgG1
Anti-Human CPS1 / Carbamoyl-Phosphate Synthetase Positive Control Sample: HeLa cells. Liver or Hepatocellular Carcinoma (HCC)
Cellular Localization of Antibody Finely granular cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human CPS1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CPS1 / Carbamoyl-Phosphate Synthetase Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Variations in CPS1 / Carbamoyl-Phosphate Synthetase antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the duodenum and small intestine and hepatocytes in liver. More moderate antibody staining intensity was present in glandular cells in the duodenum and small intestine and hepatocytes in liver. Low, but measureable presence of CPS1 / Carbamoyl-Phosphate Synthetase could be seen in. We were unable to detect CPS1 / Carbamoyl-Phosphate Synthetase in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Type:

Tissues from cancer patients, for instance, have their own distinct pattern of CPS1 / Carbamoyl-Phosphate Synthetase expression as measured by anti-CPS1 / Carbamoyl-Phosphate Synthetase antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - ++ - - - - - - - - - - - -
CPS1 Variability + + + + + + + ++ + + + + ++ + + + ++ + + +

Limitations and Warranty

This product is for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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