Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/198)
Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/198)Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/198)Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/198)Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/198)

Anti-Cyclin B1 Antibody Clone CCNB1/1098

$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 891-MSM2
Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
891-MSM2-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 891-MSM2-P1
Cyclin B1 General Information
Alternate Names
G2/mitotic-specific cyclin-B1, CCNB1
Molecular Weight
55-62kDa
Chromosomal Location
5q13.2
Curated Database and Bioinformatic Data
Gene SymbolCCNB1
Entrez Gene ID891
Ensemble Gene IDENSG00000134057
RefSeq Protein Accession(s)NP_001341773, NP_001341774, NP_114172
RefSeq mRNA Accession(s)NM_001354844, NM_001354845, NM_031966
RefSeq Genomic Accession(s)NC_000005, NC_018916,
UniProt ID(s)P14635
UniGene ID(s)P14635
HGNC ID(s)1579
Cosmic ID(s)CCNB1
KEGG Gene ID(s)hsa:891
PharmGKB ID(s)PA95
General Description of Cyclin B1.
It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr, 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr, 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent, is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Human and Mouse Anti-Cyclin B1 Antibody Product Attributes

Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cyclin B1 Antibody Clone: CCNB1/1098
Clone CCNB1/1098 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Mouse Cyclin B1 Positive Control Sample: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.
Cellular Localization of Antibody Cytoplasmic & Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human full-length CCNB1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Cyclin B1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CCNB1 antibody staining is expected to be primarily localized to the cytosol. There is variability in either the signal strength or the localization of signal in cytosol from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in Cyclin B1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the seminiferous ducts in testis, germinal center cells in the lymph node and tonsil and hematopoietic cells in the bone marrow. More moderate antibody staining intensity was present in cells in the seminiferous ducts in testis, germinal center cells in the lymph node and tonsil and hematopoietic cells in the bone marrow. Low, but measureable presence of Cyclin B1 could be seen in cells in the endometrial stroma in endometrium, cells in the tubules in kidney, glandular cells in the cervix, uterine and endometrium, Langerhans in skin, lymphoid tissue in appendix, melanocytes in skin, squamous epithelial cells in the cervix and uterine. We were unable to detect Cyclin B1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Cyclin B1 expression as measured by anti-Cyclin B1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++
CCNB1 Variability ++ ++ + ++ + ++ ++ ++ ++ + + ++ + + ++ + ++ ++ ++ +

Limitations and Warranty

enQuire Bio's Cyclin B1 Anti-Human, Mouse Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Cyclin B1 Antibody Clone CCNB1/1098

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