Flow Cytometry of human Cyclin D1 on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Monoclonal Antibody (DCS-6).
Flow Cytometry of human Cyclin D1 on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Monoclonal Antibody (DCS-6).

Anti-Cyclin D1 Antibody Clone DCS-6

$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 595-MSM1
Species: Human, Monkey, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
595-MSM1-P1 Size: 100 ug, Tag: Unconjugated
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: 595-MSM1-P1
Cyclin D1 General Information
Alternate Names
Cyclin-D1CCND1
Molecular Weight
36kDa
Chromosomal Location
11q13.2
Curated Database and Bioinformatic Data
Gene SymbolCCND1
Entrez Gene ID595
Ensemble Gene IDENSG00000110092
RefSeq Protein Accession(s)NP_444284
RefSeq mRNA Accession(s)NM_053056,
RefSeq Genomic Accession(s)NC_000011, NC_018922, NG_007375
UniProt ID(s)Q6FI00, P24385
UniGene ID(s)Q6FI00, P24385
HGNC ID(s)1582
Cosmic ID(s)CCND1
KEGG Gene ID(s)hsa:595
PharmGKB ID(s)PA75
General Description of Cyclin D1.
Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL, follicular lymphomas (cyclin D1 negative). About 40% of breast carcinomas are positive for Cyclin D1. Occasionally, hairy cell leukemia, plasma cell myeloma weakly express Cyclin D1.

Human, Monkey, Mouse, and Rat Anti-Cyclin D1 Antibody Product Attributes

Species: Human, Monkey, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Cyclin D1 Antibody Clone: DCS-6
Clone DCS-6 Host and Isotype: Mouse IgG2a kappa
Anti-Human, Monkey, Mouse, and Rat Cyclin D1 Positive Control Sample: T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. Mantle cell lymphoma, breast or bladder carcinomas.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human full length recombinant cyclin D1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Cyclin D1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CCND1 antibody staining is expected to be primarily localized to the nucleoplasm. There is variability in either the signal strength or the localization of signal in nucleoplasm from cell to cell.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Cyclin D1 expression as measured by anti-Cyclin D1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + + - - - - + + + - + - - + + + + - + -
CCND1 Variability +++ ++ + ++ + + ++ +++ ++ ++ ++ ++ ++ +++ ++ ++ ++ + ++ ++

Limitations and Warranty

enQuire Bio's Cyclin D1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “Anti-Cyclin D1 Antibody Clone DCS-6”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

Anti-Cyclin D1 Antibody Clone DCS-6

https://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-595-MSM1-P1-anti-Cyclin-D1-antibody.jpeg