Formalin-fixed, paraffin-embedded Human Heart stained with Cytochrome C Monoclonal Antibody (CTC5).
Formalin-fixed, paraffin-embedded Human Heart stained with Cytochrome C Monoclonal Antibody (CTC5).

Anti-Cytochrome C Antibody Clone 7H8.2C12

$ 298.00
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SKU: 54205-MSM3
Species: Human, Mouse, Rat, Horse, Canine, Pigeon, Frog, and Drosophila
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
54205-MSM3-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
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SKU: 54205-MSM3-P1
Cytochrome C General Information
Alternate Names
Cytochrome C, Cytochrome Complex, CYCS
Molecular Weight
15kDa
Chromosomal Location
7p15.3
Curated Database and Bioinformatic Data
Gene SymbolCYCS
Entrez Gene ID54205
Ensemble Gene IDENSG00000172115
RefSeq Protein Accession(s)NP_061820
RefSeq mRNA Accession(s)NM_018947
RefSeq Genomic Accession(s)NG_023438, NC_000007, NC_018918
UniProt ID(s)P99999, Q96BV4, P00001, G4XXL9
UniGene ID(s)P99999, Q96BV4, P00001, G4XXL9
HGNC ID(s)19986
Cosmic ID(s)CYCS
KEGG Gene ID(s)hsa:54205
PharmGKB ID(s)PA134981636
General Description of Cytochrome C.
Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c, to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

Human, Mouse, Rat, Horse, Canine, Pigeon, Frog, and Drosophila Anti-Cytochrome C Antibody Product Attributes

Species: Human, Mouse, Rat, Horse, Canine, Pigeon, Frog, and Drosophila
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Cytochrome C Antibody Clone: CTC05
Clone CTC05 Host and Isotype: Mouse IgG2b kappa
Anti-Human, Mouse, Rat, Horse, Canine, Pigeon, Frog, and Drosophila Cytochrome C Positive Control Sample: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant cytochrome c protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Cytochrome C Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CYCS antibody staining is expected to be primarily localized to the mitochondria.

Observed Antibody Staining Data By Tissue Type:

Variations in Cytochrome C antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the seminiferous ducts in testis, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the appendix, breast, colon, duodenum, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine and stomach, hematopoietic cells in the bone marrow, islets of Langerhans in pancreas, non-germinal center cells in the lymph node, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in cells in the seminiferous ducts in testis, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the appendix, breast, colon, duodenum, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine and stomach, hematopoietic cells in the bone marrow, islets of Langerhans in pancreas, non-germinal center cells in the lymph node, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. Low, but measureable presence of Cytochrome C could be seen inadipocytes in breast and mesenchymal tissue, bile duct cells in the liver, cells in the endometrial stroma in endometrium, cells in the red pulp in spleen, cells in the white pulp in spleen, chondrocytes in mesenchymal tissue, endothelial cells in the cerebral cortex and colon, fibroblasts in skin and mesenchymal tissue, follicle cells in the ovary, glial cells in the caudate nucleus, cerebral cortex and hippocampus, melanocytes in skin, neuropil in cerebral cortex, ovarian stroma cells in the ovary, peripheral nerve in mesenchymal tissue and pneumocytes in lung. We were unable to detect Cytochrome C in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Cytochrome C expression as measured by anti-Cytochrome C antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ ++ +++ +++ ++ ++ +++ +++ +++ +++ +++ +++ +++ +++ ++ ++ +++ +++ ++ ++
CYCS Variability + ++ ++ + ++ ++ ++ + + ++ + + ++ ++ ++ + + + ++ ++

Limitations and Warranty

enQuire Bio's Cytochrome C Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Cytochrome C Antibody Clone 7H8.2C12

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