Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin 1/13 Monoclonal Antibody (DE-K13)

Human and Cat Anti-Cytokeratin 10/13 Antibody Clone DE-K13

$ 359.00
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SKU: MSM11-606
Species: Human and Cat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: MSM11-606-P1
Cytokeratin 10/13 General Information
Alternate Names
Keratin, type I cytoskeletal 10, cytokeratin-10, CK-10, keratin-10, K10, KRT10
Molecular Weight
56.5kDa (CK10) & 53kDa (CK13)
Chromosomal Location
17q21.2
Curated Database and Bioinformatic Data
Gene SymbolKRT10
Entrez Gene ID3858
Ensemble Gene IDENSG00000186395
RefSeq Protein Accession(s)XP_005257400, NP_000412
RefSeq mRNA Accession(s)NM_000421, XM_005257343
RefSeq Genomic Accession(s)NC_018928, NG_033147, NG_008405, NC_000017
UniProt ID(s)P13645
UniGene ID(s)P13645
HGNC ID(s)6413
Cosmic ID(s)KRT10
KEGG Gene ID(s)hsa:3858
PharmGKB ID(s)PA30200
General Description of Cytokeratin 10/13.
This antibody recognizes cytokeratin 10 (56.5kDa), cytokeratin 13 (53kDa) in Western Blot. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying, non-cornifying stratified epithelia, more differentiated cells of squamous carcinomas.

Human and Cat Anti-Cytokeratin 10/13 Antibody Product Attributes

Species: Human and Cat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.25-0.5ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Cytokeratin 10/13 Antibody Clone: DE-K13
Clone DE-K13 Host and Isotype: Mouse IgG2a kappa
Anti-Human and Cat Cytokeratin 10/13 Positive Control Sample: A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 1.0mg/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Cytokeratin 10/13 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in Cytokeratin 10/13 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in epidermal cells in the skin and keratinocytes in skin. More moderate antibody staining intensity was present in epidermal cells in the skin and keratinocytes in skin. Low, but measureable presence of Cytokeratin 10/13 could be seen in. We were unable to detect Cytokeratin 10/13 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's Cytokeratin 10/13 Anti-Human, Cat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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