Human and Mouse Anti-Cytokeratin 10 Antibody Product Attributes
Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.1-0.2ug of antibody/ml for 30 min at RT)
Anti-Cytokeratin 10 Antibody Clone: SPM261
Clone SPM261 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Mouse Cytokeratin 10 Positive Control Sample: A431, HeLa, MCF7 cells or Esophagus.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Skin extract of a human Psoriasis patient
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
Cytokeratin 10 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in Cytokeratin 10 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in epidermal cells in the skin and keratinocytes in skin. More moderate antibody staining intensity was present in epidermal cells in the skin and keratinocytes in skin. Low, but measureable presence of Cytokeratin 10 could be seen in. We were unable to detect Cytokeratin 10 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's Cytokeratin 10 Anti-Human, Mouse Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.