Human Anti-Cytokeratin 15 Antibody Product Attributes
Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-Cytokeratin 15 Antibody Clone: LHK15
Clone LHK15 Host and Isotype: Mouse IgG2a kappa
Anti-Human Cytokeratin 15 Positive Control Sample: A431 cells. Skin or Colon
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A 17 amino acids synthetic peptide from the C- terminal of Human Cytokeratin 15
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Cytokeratin 15 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-KRT15 antibody staining is expected to be primarily localized to the intermediate filaments. There is variability in either the signal strength or the localization of signal in intermediate filaments from cell to cell.Observed Antibody Staining Data By Tissue Type:
Variations in Cytokeratin 15 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the breast, respiratory epithelial cells in the bronchus and squamous epithelial cells in the esophagus. More moderate antibody staining intensity was present in glandular cells in the breast, respiratory epithelial cells in the bronchus and squamous epithelial cells in the esophagus. Low, but measureable presence of Cytokeratin 15 could be seen inglandular cells in the prostate. We were unable to detect Cytokeratin 15 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Cytokeratin 15 expression as measured by anti-Cytokeratin 15 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - |
KRT15 Variability | + | + | ++ | + | + | + | ++ | + | ++ | + | + | + | + | + | + | ++ | + | + | + | ++ |
Limitations and Warranty
enQuire Bio's Cytokeratin 15 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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