Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
|Recommended Dilution (Conc)||1:25-1:75|
|Pretreatment||Citrate Buffer pH 6.0|
|Incubation Parameters||30 min at Room Temperature|
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Anti-Cytokeratin 15 Antibody Clone: LKH15
Host and Isotype: Mouse IgG2a, kappa
Recommended Positive Control Sample: Skin
Cellular Localization of Antibody LKH15 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A 17-mer synthetic peptide from the C-terminus of human cytokeratin 15.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Cytokeratin 15 Information for Pathologists
Summary:Molecular weight of 50 kDa. Downregulated in activated keratinocytes in psoriasis, hypertrophic scars and skin injury (J Invest Dermatol 1999;112:362, Exp Cell Res 2000;254:80). Uses by pathologists No significant clinical use by pathologists. Positive staining - normal
Common Uses By Pathologists:No significant clinical use by pathologists. Positive staining - normal Conjunctiva (basal cells) and cornea limbus epithelial cells (basal and suprabasal cells, Invest Ophthalmol Vis Sci 2006;47:4780). Esophagus-Mol Cell Biol 2004;24:3168). Fetal epidermis and nail, hair follicle bulge and follicular stem cells (Clin Exp Dermatol 2006;31:807).
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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