Immunohistochemical staining of Cytokeratin 15  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Cytokeratin 15 [LKH15] Antibody (cGMP).

$ 135.00$ 425.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD112
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG2a, kappa

Product NumberDescriptionPrice
QIVD112-0.1ml Size: 0.1 ml, Format: Concentrate $ 158.00
QIVD112-0.5ml Size: 0.5 ml, Format: Concentrate $ 288.00
QIVD112-1ml Size: 1 ml, Format: Concentrate $ 478.00
Shipping Information
In Stock
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: QIVD112-0.1ml
Cytokeratin 15 General Information
Alternate Names
Molecular Weight
49.2 kDa
Chromosomal Location
q21.2 [chr: 17] [chr_start: 41513745] [chr_end: 41522529] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolKRT15
Entrez Gene ID3866
RefSeq Protein Accession(s)NP_002266
RefSeq mRNA Accession(s); XM_011524784; XM_017024614; NM_002275
RefSeq Genomic Accession(s)NG_012284; NC_000017
UniProt ID(s)P19012
PharmGKB ID(s)PA30208
KEGG Gene ID(s)hsa:3866
General Description of Cytokeratin 15 .
Cytokeratin 15 is a type I cytokeratin without a defined type II partner. Cytokeratin 15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:75
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Cytokeratin 15 Antibody Clone: LKH15
Host and Isotype: Mouse IgG2a, kappa
Recommended Positive Control Sample: Skin
Cellular Localization of Antibody LKH15 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A 17-mer synthetic peptide from the C-terminus of human cytokeratin 15.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Cytokeratin 15 Information for Pathologists

Summary:

Molecular weight of 50 kDa. Downregulated in activated keratinocytes in psoriasis, hypertrophic scars and skin injury (J Invest Dermatol 1999;112:362, Exp Cell Res 2000;254:80). Uses by pathologists No significant clinical use by pathologists. Positive staining - normal

Common Uses By Pathologists:

No significant clinical use by pathologists. Positive staining - normal Conjunctiva (basal cells) and cornea limbus epithelial cells (basal and suprabasal cells, Invest Ophthalmol Vis Sci 2006;47:4780). Esophagus-Mol Cell Biol 2004;24:3168). Fetal epidermis and nail, hair follicle bulge and follicular stem cells (Clin Exp Dermatol 2006;31:807).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

There are no reviews yet.

Be the first to review “Cytokeratin 15 [LKH15] Antibody (cGMP).”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Chat: See lower right corner. | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

Cytokeratin 15 [LKH15] Antibody (cGMP).