Immunohistochemical staining of Cytokeratin 16  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Cytokeratin 16 [LL025] Antibody (cGMP).

$ 135.00$ 395.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD119
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1

Product NumberDescriptionPrice
QIVD119-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD119-0.5ml Size: 0.5 ml, Format: Concentrate $ 235.00
QIVD119-1ml Size: 1 ml, Format: Concentrate $ 395.00
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SKU: QIVD119-0.1ml
Cytokeratin 16 General Information
Alternate Names
Molecular Weight
51.3 kDa
Chromosomal Location
q21.2 [chr: 17] [chr_start: 41609778] [chr_end: 41615899] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolKRT16
Entrez Gene ID3868
RefSeq Protein Accession(s)NP_005548
RefSeq mRNA Accession(s); NM_005557
RefSeq Genomic Accession(s)NG_008301; NC_000017
UniProt ID(s)P08779
PharmGKB ID(s)PA30210
KEGG Gene ID(s)hsa:3868
Associated Diseases (KEGG IDs)Pachyonychia congenita 1 (PC1) [MIM:167200]: An autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma, follicular hyperkeratosis, and oral leukokeratosis. Hyperhidrosis of the hands and feet is usually present. {ECO:0000269|PubMed:10521820, ECO:0000269|PubMed:10606845, ECO:0000269|PubMed:10839714, ECO:0000269|PubMed:11359398, ECO:0000269|PubMed:11886499, ECO:0000269|PubMed:16250206, ECO:0000269|PubMed:17719747, ECO:0000269|PubMed:21160496, ECO:0000269|PubMed:21326300, ECO:0000269|PubMed:22668561, ECO:0000269|PubMed:24118415, ECO:0000269|PubMed:7539673}. The disease is caused by mutations affecting the gene represented in this entry.; Keratoderma, palmoplantar, non-epidermolytic, focal 1 (FNEPPK1) [MIM:613000]: A dermatological disorder characterized by non-epidermolytic palmoplantar keratoderma limited to the pressure points on the balls of the feet, with later mild involvement on the palms. Oral, genital and follicular keratotic lesions are often present. {ECO:0000269|PubMed:8595410}. The disease is caused by mutations affecting the gene represented in this entry.; KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.
General Description of Cytokeratin 16 .
This antibody is specific to a 48 kDa protein, which is identified as cytokeratin 16. Cytokeratin 16 is expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region. High concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:75
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Cytokeratin 16 Antibody Clone: LL025
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Skin, Squamous Cell Carcinoma
Cellular Localization of Antibody LL025 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with a synthetic peptide from the C-terminus of human cytokeratin 16.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Cytokeratin 16 Information for Pathologists

Summary:

Molecular weight of 48 kDa. Paired with keratin 6. Activated keratinocytes produce keratin 6, 16, 17, not normally present (Am J Pathol 1998;152:1133). Upregulated in benign keratinocyte hyperplasia, including psoriasis; prepsoriatic skin may be converted to a distinct adult tissue type resembling hard palate (J Investig Dermatol Symp Proc 2006;11:16). Also upregulated in stress incontinence in periurethral vaginal wall (Hum Reprod 2006;21:22), conjunctival epithelium in Sjogren’s syndrome (Exp Eye Res 2003;77:17).

Common Uses By Pathologists:

No significant clinical use by pathologists. Positive staining - normal Esophagus. Hair, nail and glands of epidermis. Melanocytes (Am J Dermatopathol 2005;27:476).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Cytokeratin 16 [LL025] Antibody (cGMP).