Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:25-1:75 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-Cytokeratin 16 Antibody Clone: LL025
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Skin, Squamous Cell Carcinoma
Cellular Localization of Antibody LL025 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with a synthetic peptide from the C-terminus of human cytokeratin 16.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Cytokeratin 16 Information for Pathologists
Summary:
Molecular weight of 48 kDa. Paired with keratin 6. Activated keratinocytes produce keratin 6, 16, 17, not normally present (Am J Pathol 1998;152:1133). Upregulated in benign keratinocyte hyperplasia, including psoriasis; prepsoriatic skin may be converted to a distinct adult tissue type resembling hard palate (J Investig Dermatol Symp Proc 2006;11:16). Also upregulated in stress incontinence in periurethral vaginal wall (Hum Reprod 2006;21:22), conjunctival epithelium in SjogrenÂs syndrome (Exp Eye Res 2003;77:17).Common Uses By Pathologists:
No significant clinical use by pathologists. Positive staining - normal Esophagus. Hair, nail and glands of epidermis. Melanocytes (Am J Dermatopathol 2005;27:476).Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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