Immunohistochemical staining of Cytokeratin 17  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Cytokeratin 17 [E3] Antibody (cGMP).

$ 135.00$ 515.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD109
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG2b, kappa

Product NumberDescriptionPrice
QIVD109-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD109-0.5ml Size: 0.5 ml, Format: Concentrate $ 305.00
QIVD109-1ml Size: 1 ml, Format: Concentrate $ 515.00
Shipping Information
In Stock
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: QIVD109-0.1ml
Cytokeratin 17 General Information
Alternate Names
Molecular Weight
48.1 kDa
Chromosomal Location
q21.2 [chr: 17] [chr_start: 41619441] [chr_end: 41624842] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolKRT17
Entrez Gene ID3872
RefSeq Protein Accession(s)NP_000413
RefSeq mRNA Accession(s); NM_000422
RefSeq Genomic Accession(s)NG_008625; NG_009090; NC_000017
UniProt ID(s)Q04695
PharmGKB ID(s)PA30214
KEGG Gene ID(s)hsa:3872
Associated Diseases (KEGG IDs)Pachyonychia congenita 2 (PC2) [MIM:167210]: An autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma and hyperhidrosis, follicular hyperkeratosis, multiple epidermal cysts, absent/sparse eyebrow and body hair, and by the presence of natal teeth. {ECO:0000269|PubMed:10571744, ECO:0000269|PubMed:11348474, ECO:0000269|PubMed:11874497, ECO:0000269|PubMed:11886499, ECO:0000269|PubMed:15102078, ECO:0000269|PubMed:15795125, ECO:0000269|PubMed:16250206, ECO:0000269|PubMed:16620218, ECO:0000269|PubMed:16625196, ECO:0000269|PubMed:17719747, ECO:0000269|PubMed:18547302, ECO:0000269|PubMed:19470054, ECO:0000269|PubMed:21326300, ECO:0000269|PubMed:23278621, ECO:0000269|PubMed:23855588, ECO:0000269|PubMed:7539673, ECO:0000269|PubMed:9008238, ECO:0000269|PubMed:9767294}. The disease is caused by mutations affecting the gene represented in this entry.; Steatocystoma multiplex (SM) [MIM:184500]: Disease characterized by round or oval cystic tumors widely distributed on the back, anterior trunk, arms, scrotum, and thighs. {ECO:0000269|PubMed:16620218, ECO:0000269|PubMed:9008238, ECO:0000269|PubMed:9767294}. The disease is caused by mutations affecting the gene represented in this entry.; KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.
General Description of Cytokeratin 17 .
This antibody is specific to 46 kDa cytokeratin protein designated cytokeratin 17. It reacts with basal cells in complex epithelia and reacts strongly with squamous cell carcinomas.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:50
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Cytokeratin 17 Antibody Clone: E3
Host and Isotype: Mouse IgG2b, kappa
Recommended Positive Control Sample: Skin
Cellular Localization of Antibody E3 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Cytoskeletal fraction of rat colon epithelium.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Cytokeratin 17 Information for Pathologists

Summary:

Molecular weight of 48 kDa. Basal type cytokeratin (also 34betaE12, CK5/6, CK14) of complex epithelia but not in stratified or simple epithelia (Eur J Cell Biol 1992;59:127). Rapidly induced in wounded stratified epithelia (also CK 6, CK16); regulates cell growth through binding to the adaptor protein 14-3-3sigma (Nature 2006;441:362). Mutations cause pachyonychia congenita type 2 (J Dermatol 2006;33:161) or steatocystoma multiplex (Br J Dermatol 1998;139:475). Uses by pathologists

Common Uses By Pathologists:

Distinguish pancreatobiliary adenocarcinoma (CK17+) from extra-pancreatobiliary nonmucinous adenocarcinoma (CK17-, Am J Surg Pathol 2005;29:359). Distinguish cholangiocarcinoma (60% are CK17+) from hepatocellular carcinoma (CK17-, J Gastrointestin Liver Dis 2006;15:9). Distinguish renal urothelial carcinoma (CK5/CK6+, CK17+, vimentin-) from collecting duct carcinoma (CK5/CK6-, CK17-, vimentin+, Am J Surg Pathol 2005;29:747). Considered a marker of or associated with the basal phenotype (also CK 5/6, CK 14) of invasive or in situ ductal carcinoma of breast (Mod Pathol 2006;19:1506). Sensitive marker of sentinel nodal metastases by RT-PCR in oral squamous cell carcinoma (Clin Cancer Res 2006;12:2498).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

There are no reviews yet.

Be the first to review “Cytokeratin 17 [E3] Antibody (cGMP).”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

Cytokeratin 17 [E3] Antibody (cGMP).