Anti-Cytokeratin 18 Anti-HumanAntibody Validation Data
Analysis of Mass Spec data (dashed-line) of fractions stained with Cytokeratin 18 Anti-Human MS-QAVA™ monoclonal antibody [Clone: DC10] (solid-line), reveals that less than 8.3% of signal is attributable to non-specific binding of anti-Cytokeratin 18 [Clone:  DC10] to targets other than KRT18 protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in A431, RT4 and MCF7 cells.

Anti-Anti-Human Antibody Clone DC10

$ 298.00
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SKU: 3875-MSM1
Species: Human
Tested Applications: Western Blot
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: MS-QAVA™ Validated

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Datasheets and Documentation
Product Datasheet
SKU: 3875-MSM1-P1
Cytokeratin 18 Anti-Human General Information
Alternate Names
Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18 Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18, anti-Cell Proliferation-inducing Gene 46 Protein antibody, anti-CK18 antibody, anti-CYK18 Cytokeratin Endo B antibody, anti-K18 antibody, anti-Keratin-18 antibody, anti-Kerd antibody, and anti-KRT18 antibody
Molecular Weight
45kDa
Chromosomal Location
12q13.13
Curated Database and Bioinformatic Data
Gene SymbolKRT18
Entrez Gene ID3875
Ensemble Gene IDENSG00000111057
RefSeq Protein Accession(s)NP_954657, NP_000215
RefSeq mRNA Accession(s)NM_199187, NM_000224
RefSeq Genomic Accession(s)NC_000012, NG_008351, NG_008402, NC_018923
UniProt ID(s)P05783, A0A024RAY2
UniGene ID(s)P05783, A0A024RAY2
HGNC ID(s)6430
Cosmic ID(s)KRT18
KEGG Gene ID(s)hsa:3875
PharmGKB ID(s)PA30217
General Description of Cytokeratin 18 Anti-Human.
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Human Anti-Cytokeratin 18 Anti-Human Antibody Product Attributes

Species: Human
Tested Applications: Western Blot. Additionally, this antibody has been validated by MS-QAVA™. Specificity has been quantitatively determined via Mass Spec.
Clonality: Monoclonal
Anti-Cytokeratin 18 Anti-Human Antibody Clone: DC10
Clone DC10 Host and Isotype: Mouse IgG1
Anti-Human Cytokeratin 18 Anti-Human Positive Control Sample: MCF-7, HeLa, A431 cells or Breast Cancer
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: PMC-42 human breast carcinoma cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Cytokeratin 18 Anti-Human Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Variations in Cytokeratin 18 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in bile duct cells in the liver, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the appendix, breast, cervix, uterine, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, hepatocytes in liver, pneumocytes in lung, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. More moderate antibody staining intensity was present in bile duct cells in the liver, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the appendix, breast, cervix, uterine, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, hepatocytes in liver, pneumocytes in lung, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. Low, but measureable presence of Cytokeratin 18 could be seen in. We were unable to detect Cytokeratin 18 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Type:

Tissues from cancer patients, for instance, have their own distinct pattern of Cytokeratin 18 expression as measured by anti-Cytokeratin 18 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ +++ ++ +++ +++ - - +++ +++ - - +++ +++ +++ ++ - +++ - +++ ++
KRT18 Variability ++ + +++ ++ + + ++ ++ ++ + + + + + ++ + + ++ + ++

Limitations and Warranty

This product is for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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