Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (Cocktail).
Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (Cocktail).Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (Cocktail).Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (Cocktail).Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (Cocktail).

Anti-Cytokeratin 18 Antibody Clone Cocktail

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 3875-MSM8
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 3875-MSM8-P1
Cytokeratin 18 General Information
Alternate Names
Keratin 18
Molecular Weight
45kDa
Chromosomal Location
12q13.13
Curated Database and Bioinformatic Data
Gene SymbolKRT18
Entrez Gene ID3875
Ensemble Gene IDENSG00000111057
RefSeq Protein Accession(s)NP_954657, NP_000215
RefSeq mRNA Accession(s)NM_199187, NM_000224
RefSeq Genomic Accession(s)NC_000012, NG_008351, NG_008402, NC_018923
UniProt ID(s)P05783, A0A024RAY2
UniGene ID(s)P05783, A0A024RAY2
HGNC ID(s)6430
Cosmic ID(s)KRT18
KEGG Gene ID(s)hsa:3875
PharmGKB ID(s)PA30217
General Description of Cytokeratin 18.
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary,, thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8, 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract, urogenital tract, as well as endocrine, exocrine tissues, mesothelial cells are positive for cytokeratin 18.

Human Anti-Cytokeratin 18 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cytokeratin 18 Antibody Clone: Cocktail
Clone Cocktail Host and Isotype: Mouse IgG
Anti-Human Cytokeratin 18 Positive Control Sample: MCF-7, HeLa, A431 cells or Breast Cancer
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human KRT18 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Cytokeratin 18 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-KRT18 antibody staining is expected to be primarily localized to the cytosol.

Observed Antibody Staining Data By Tissue Type:

Variations in Cytokeratin 18 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in bile duct cells in the liver, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the appendix, breast, cervix, uterine, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, hepatocytes in liver, pneumocytes in lung, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. More moderate antibody staining intensity was present in bile duct cells in the liver, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the appendix, breast, cervix, uterine, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, hepatocytes in liver, pneumocytes in lung, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. Low, but measureable presence of Cytokeratin 18 could be seen in. We were unable to detect Cytokeratin 18 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Cytokeratin 18 expression as measured by anti-Cytokeratin 18 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ +++ ++ +++ +++ - - +++ +++ - - +++ +++ +++ ++ - +++ - +++ ++
KRT18 Variability ++ + +++ ++ + + ++ ++ ++ + + + + + ++ + + ++ + ++

Limitations and Warranty

enQuire Bio's Cytokeratin 18 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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