Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/1198)
Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/1198)Formalin-fixed, paraffin-embedded Rat Lung stained with Cytokeratin 7 Monoclonal Antibody (KRT7/1198)

Anti-Cytokeratin 7 Antibody Clone KRT7/1198

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 3855-MSM8
Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

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In Stock: 1-2 Day Lead Time.
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Datasheets and Documentation
Product Datasheet
SKU: 3855-MSM8-P1
Cytokeratin 7 General Information
Alternate Names
Keratin, type II cytoskeletal 7, cytokeratin-7, CK-7, keratin-7, K7, sarcolectin, SCL, KRT7
Molecular Weight
55kDa
Chromosomal Location
12q13.13
Curated Database and Bioinformatic Data
Gene SymbolKRT7
Entrez Gene ID3855
Ensemble Gene IDENSG00000135480
RefSeq Protein Accession(s)NP_005547, XP_016874783, XP_011536627
RefSeq mRNA Accession(s)XR_001748699, NM_005556, XR_001748700, XM_017019294
RefSeq Genomic Accession(s)NC_000012, NG_012296, NC_018923
UniProt ID(s)P08729
UniGene ID(s)P08729
HGNC ID(s)6445
Cosmic ID(s)KRT7
KEGG Gene ID(s)hsa:3855
PharmGKB ID(s)PA30233
General Description of Cytokeratin 7.
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7, shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular, transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung,, breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Human and Rat Anti-Cytokeratin 7 Antibody Product Attributes

Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Cytokeratin 7 Antibody Clone: KRT7/1198
Clone KRT7/1198 Host and Isotype: Mouse IgG1
Anti-Human and Rat Cytokeratin 7 Positive Control Sample: HeLa cells. Carcinoma of ovary, lung, cervix, or breast
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human KRT7 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Cytokeratin 7 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in Cytokeratin 7 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the breast, cervix, uterine, endometrium, fallopian tube, gallbladder, salivary gland, seminal vesicle and thyroid gland, respiratory epithelial cells in the bronchus and nasopharynx, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in glandular cells in the breast, cervix, uterine, endometrium, fallopian tube, gallbladder, salivary gland, seminal vesicle and thyroid gland, respiratory epithelial cells in the bronchus and nasopharynx, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. Low, but measureable presence of Cytokeratin 7 could be seen inglandular cells in the parathyroid gland and stomach and myoepithelial cells in the breast. We were unable to detect Cytokeratin 7 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Cytokeratin 7 expression as measured by anti-Cytokeratin 7 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ - +++ - ++ - - + +++ - - +++ +++ + - - +++ - +++ +++
KRT7 Variability ++ + ++ + ++ + + ++ + + + + + +++ + ++ ++ + + +

Limitations and Warranty

enQuire Bio's Cytokeratin 7 Anti-Human, Rat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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