Immunohistochemical staining of Cytokeratin 7  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Cytokeratin 7 [OV-TL 12/30] Antibody (cGMP).

$ 135.00$ 485.00
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Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1, kappa

Product NumberDescriptionPrice
QIVD111-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD111-0.5ml Size: 0.5 ml, Format: Concentrate $ 285.00
QIVD111-1ml Size: 1 ml, Format: Concentrate $ 485.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:


Bioactivity (test results eg. IU/ml):

SKU: QIVD111-0.1ml
Cytokeratin 7 General Information
Alternate Names
Molecular Weight
51.4 kDa
Chromosomal Location
q13.13 [chr: 12] [chr_start: 52232520] [chr_end: 52252186] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolKRT7
Entrez Gene ID3855
RefSeq Protein Accession(s)NP_005547
RefSeq mRNA Accession(s)XR_001748699; XM_011538325; NM_005556; XR_001748700; XM_017019294
RefSeq Genomic Accession(s)NC_000012; NG_012296
UniProt ID(s)P08729
PharmGKB ID(s)PA30233
KEGG Gene ID(s)hsa:3855
General Description of Cytokeratin 7 .
This antibody recognizes keratin polypeptide of 54 kDa. It stains a number of epithelial cells including ductal and glandular epithelia. Stratified squamous epithelia are positive but transitional epithelium of the urinary tract does not show any staining. This antibody labels adenocarcinomas and transitional carcinomas but does not react with squamous cell carcinomas.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Cytokeratin 7 Antibody Clone: OV-TL 12/30
Host and Isotype: Mouse IgG1, kappa
Recommended Positive Control Sample: Skin, Lung
Cellular Localization of Antibody OV-TL 12/30 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were immunized with ovarian carcinoma cell line, OTN11.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Cytokeratin 7 Information for Pathologists


Type II keratin of simple nonkeratinizing epithelia; molecular weight 51.4 kDa (OMIM: 148059 [Accessed 14 September 2018]). Expression similar to but more limited than keratins 8 and 18 in simple, pseudostratified and ductal epithelium, mesothelium and urothelium. Essential features Membranous / cytoplasmic marker with expression in many normal epithelia and epithelial tumors. Despite wide distribution, useful as part of a panel in determining primary site of metastatic carcinoma.

Common Uses By Pathologists:

CK7 / CK20 profiles (general patterns, specificity varies):. CK7+ / CK20+: Extrahepatic carcinoma of the bile duct (majority) (Pathol Res Pract 2003;199:65). Gastric adenocarcinoma, subset (majority are CK7+ / CK20-) . Pancreatic adenocarcinoma, minor subset (co-expression associated with worse prognosis, however, majority are CK7+ / CK20-) (Cancer 2006;106:693).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Cytokeratin 7 [OV-TL 12/30] Antibody (cGMP).