Immunohistochemical staining of Cytokeratin 7  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Cytokeratin 7 [LP1K ] Antibody (cGMP).

$ 158.00$ 708.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD113
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1

Product NumberDescriptionPrice
QIVD113-0.1ml Size: 0.1 ml, Format: Concentrate $ 128.00
QIVD113-0.5ml Size: 0.5 ml, Format: Concentrate $ 258.00
QIVD113-1ml Size: 1.0 ml, Format: Concentrate $ 438.00
QIVD113-6ml Size: 6 ml, Format: Predilute $ 238.00
QIVD113-25ml Size: 25 ml, Format: Predilute $ 568.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

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SKU: QIVD113-0.1ml
Cytokeratin 7 General Information
Alternate Names
Molecular Weight
51.4 kDa
Chromosomal Location
q13.13 [chr: 12] [chr_start: 52232520] [chr_end: 52252186] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolKRT7
Entrez Gene ID3855
RefSeq Protein Accession(s)NP_005547
RefSeq mRNA Accession(s)XR_001748699; XM_011538325; NM_005556; XR_001748700; XM_017019294
RefSeq Genomic Accession(s)NC_000012; NG_012296
UniProt ID(s)P08729
PharmGKB ID(s)PA30233
KEGG Gene ID(s)hsa:3855
General Description of Cytokeratin 7 .
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratin 7 is expressed in epithelial cells of ovary, lung and breast. It is often used in conjunction with cytokeratin 20 and CDX-2 in distinguishing pulmonary, ovarian and breast carcinomas (CK7+) from most colon carcinomas (CK7-).

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment HistoZyme
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Cytokeratin 7 Antibody Clone: LP1K
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody LP1K Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Sonicated cytoskeleton fractions from SV40 transformed neonatal keratinocytes
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Cytokeratin 7 Information for Pathologists

Summary:

Type II keratin of simple nonkeratinizing epithelia; molecular weight 51.4 kDa (OMIM: 148059 [Accessed 14 September 2018]). Expression similar to but more limited than keratins 8 and 18 in simple, pseudostratified and ductal epithelium, mesothelium and urothelium. Essential features Membranous / cytoplasmic marker with expression in many normal epithelia and epithelial tumors. Despite wide distribution, useful as part of a panel in determining primary site of metastatic carcinoma.

Common Uses By Pathologists:

CK7 / CK20 profiles (general patterns, specificity varies):. CK7+ / CK20+: Extrahepatic carcinoma of the bile duct (majority) (Pathol Res Pract 2003;199:65). Gastric adenocarcinoma, subset (majority are CK7+ / CK20-) . Pancreatic adenocarcinoma, minor subset (co-expression associated with worse prognosis, however, majority are CK7+ / CK20-) (Cancer 2006;106:693).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Cytokeratin 7 [LP1K ] Antibody (cGMP).