Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Cytokeratin 8 Monoclonal Antibody (SPM192).
Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Cytokeratin 8 Monoclonal Antibody (SPM192).

Anti-Cytokeratin 8 Antibody Clone SPM192

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 3856-MSM4X
Species: Human and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 3856-MSM4X-P1
Cytokeratin 8 General Information
Alternate Names
Keratin, type II cytoskeletal 8, cytokeratin-8, CK-8, keratin-8, K8, KRT8
Molecular Weight
52.5kDa
Chromosomal Location
12q13.13
Curated Database and Bioinformatic Data
Gene SymbolKRT8
Entrez Gene ID3856
Ensemble Gene IDENSG00000170421
RefSeq Protein Accession(s)NP_001243211, NP_002264, NP_001243222
RefSeq mRNA Accession(s)NR_045962 NM_001256282, NM_001256293, NM_002273
RefSeq Genomic Accession(s)NC_000012, NC_018923, NG_008402
UniProt ID(s)P05787, Q7L4M3
UniGene ID(s)P05787, Q7L4M3
HGNC ID(s)6446
Cosmic ID(s)KRT8
KEGG Gene ID(s)hsa:3856
PharmGKB ID(s)PA30234
General Description of Cytokeratin 8.
Epitope of this MAb is located between aa343-357. Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins, exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia, is present in majority of adenocarcinomas, ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8, 18. CK8 exists on several types of normal, neoplastic epithelia, including many ductal, glandular epithelia such as colon, stomach, small intestine, trachea,, esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma,, adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18, CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (ring-like, perinuclear) from ductal (peripheral-predominant) carcinoma of the breast.

Human and Rat (-) Anti-Cytokeratin 8 Antibody Product Attributes

Species: Human and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cytokeratin 8 Antibody Clone: SPM192
Clone SPM192 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Rat (-) Cytokeratin 8 Positive Control Sample: MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Keratin preparation from a human carcinoma
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Cytokeratin 8 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-KRT8 antibody staining is expected to be primarily localized to the intermediate filaments.

Observed Antibody Staining Data By Tissue Type:

Variations in Cytokeratin 8 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in bile duct cells in the liver, exocrine glandular cells in the pancreas, follicle cells in the ovary, glandular cells in the appendix, breast, cervix, uterine, duodenum, endometrium, epididymis, fallopian tube, gallbladder, prostate, rectum, seminal vesicle, small intestine, stomach and thyroid gland, respiratory epithelial cells in the bronchus and nasopharynx, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in bile duct cells in the liver, exocrine glandular cells in the pancreas, follicle cells in the ovary, glandular cells in the appendix, breast, cervix, uterine, duodenum, endometrium, epididymis, fallopian tube, gallbladder, prostate, rectum, seminal vesicle, small intestine, stomach and thyroid gland, respiratory epithelial cells in the bronchus and nasopharynx, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. Low, but measureable presence of Cytokeratin 8 could be seen in. We were unable to detect Cytokeratin 8 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Cytokeratin 8 expression as measured by anti-Cytokeratin 8 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ +++ +++ +++ +++ - - +++ ++ - - +++ +++ +++ ++ - +++ + +++ +++
KRT8 Variability + + ++ + ++ + ++ + ++ + + ++ + + ++ ++ + ++ + ++

Limitations and Warranty

enQuire Bio's Cytokeratin 8 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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