Western Blot of A431 Cells using Cytokeratin, LMW Monoclonal Antibody (AE-1).

Anti-Cytokeratin, Acidic Antibody Clone AE-1

Name: Anti-Cytokeratin, Acidic Antibody Clone AE-1
Brand: enQuire Bio
Price: 429 USD
Availability: InStock

$ 429.00

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SKU: MSM4-253
Species: Human, Monkey, Cow, Canine, Rabbit, Mouse, Rat, Chicken, and Turtle
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
MSM4-253-P1	Size: 100 ug, Tag: Unconjugated

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In Stock.

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Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: MSM4-253-P1

Cytokeratin, Acidic General Information
Alternate Names
KRT77, Keratin 77
Molecular Weight
40-56.5kDa
Chromosomal Location
17q21.2
Curated Database and Bioinformatic Data
Gene Symbol	KRT77
Entrez Gene ID	374454
Ensemble Gene ID	ENSG00000189182
RefSeq Protein Accession(s)	NP_778253, XP_011536591, XP_011536590
RefSeq mRNA Accession(s)	XM_011538288, NM_175078
RefSeq Genomic Accession(s)	NC_000012, NC_018923, NG_012322
UniProt ID(s)	Q7Z794, Q0IIN1
UniGene ID(s)	Q7Z794, Q0IIN1
HGNC ID(s)	20411
Cosmic ID(s)	KRT77
KEGG Gene ID(s)	hsa:374454
PharmGKB ID(s)	PA134896049
General Description of Cytokeratin, Acidic.
This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50, 48, 46, 45,, 40kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56,, 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research, tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer,, esophageal cancer.

Human, Monkey, Cow, Canine, Rabbit, Mouse, Rat, Chicken, and Turtle Anti-Cytokeratin, Acidic Antibody Product Attributes

Species: Human, Monkey, Cow, Canine, Rabbit, Mouse, Rat, Chicken, and Turtle

Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).

Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 min at RT)

Clonality: Monoclonal

Anti-Cytokeratin, Acidic Antibody Clone: AE-1

Clone AE-1 Host and Isotype: Mouse IgG1 kappa

Anti-Human, Monkey, Cow, Canine, Rabbit, Mouse, Rat, Chicken, and Turtle Cytokeratin, Acidic Positive Control Sample: Skin, Squamous cell carcinoma (SCC)

Cellular Localization of Antibody Cytoplasmic

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.

Antibody Concentration: 200ug/ml

Antibody Purification Method:Protein A/G Purified

Immunogen: Human epidermal keratin

Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.

Cytokeratin, Acidic Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-KRT77 antibody staining is expected to be primarily localized to the nuclear membrane and plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in Cytokeratin, Acidic antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in keratinocytes in skin and squamous epithelial cells in the oral mucosa. More moderate antibody staining intensity was present in keratinocytes in skin and squamous epithelial cells in the oral mucosa. Low, but measureable presence of Cytokeratin, Acidic could be seen inepidermal cells in the skin, Langerhans in skin, squamous epithelial cells in the cervix and uterine and esophagus. We were unable to detect Cytokeratin, Acidic in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Cytokeratin, Acidic expression as measured by anti-Cytokeratin, Acidic antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
KRT77 Variability	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	++	+	++	+	++

Limitations and Warranty

enQuire Bio's Cytokeratin, Acidic Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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