Human, Rat, and Broad Species Reactivity Anti-Cytokeratin, Acidic Antibody Product Attributes
Species: Human, Rat, and Broad Species Reactivity
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cytokeratin, Acidic Antibody Clone: KRTL/1077
Clone KRTL/1077 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Rat, and Broad Species Reactivity Cytokeratin, Acidic Positive Control Sample: Skin, Squamous cell carcinoma (SCC)
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human KRT77 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Cytokeratin, Acidic Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-KRT77 antibody staining is expected to be primarily localized to the nuclear membrane and plasma membrane.Observed Antibody Staining Data By Tissue Type:
Variations in Cytokeratin, Acidic antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in keratinocytes in skin and squamous epithelial cells in the oral mucosa. More moderate antibody staining intensity was present in keratinocytes in skin and squamous epithelial cells in the oral mucosa. Low, but measureable presence of Cytokeratin, Acidic could be seen inepidermal cells in the skin, Langerhans in skin, squamous epithelial cells in the cervix and uterine and esophagus. We were unable to detect Cytokeratin, Acidic in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Cytokeratin, Acidic expression as measured by anti-Cytokeratin, Acidic antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
KRT77 Variability | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | ++ | + | ++ | + | ++ |
Limitations and Warranty
enQuire Bio's Cytokeratin, Acidic Anti-Human, Rat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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