Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, HMW Monoclonal Antibody (AE-3).
Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, HMW Monoclonal Antibody (AE-3).

Anti-Cytokeratin Antibody Clone AE-3

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: MSM5-257
Species: Human, Monkey, Cow, Canine, Rabbit, Mouse, Rat, and Chicken
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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In Stock: 1-2 Day Lead Time.
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Datasheets and Documentation
Product Datasheet
SKU: MSM5-257-P1
Cytokeratin General Information
Alternate Names
KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9, anti-KRT2B antibody, anti-KRT2P antibody, anti-HUMCYT2A antibody, anti-Keratin antibody, anti-type II Cytoskeletal 2 oral antibody, anti-K76 antibody, anti-Keratin 2p (K2P) antibody, anti-Keratin-76 antibody, anti-Cytokeratin-2P (CK-2P antibody, anti-Type-II Keratin Kb9 antibody
Molecular Weight
52-67kDa
Chromosomal Location
12q13.13
Curated Database and Bioinformatic Data
Gene SymbolKRT76
Entrez Gene ID51350
Ensemble Gene IDENSG00000185069
RefSeq Protein Accession(s)NP_056932
RefSeq mRNA Accession(s)NM_015848,
RefSeq Genomic Accession(s)NG_012420, NC_000012, NC_018923
UniProt ID(s)Q01546
UniGene ID(s)Q01546
HGNC ID(s)24430
Cosmic ID(s)KRT76
KEGG Gene ID(s)hsa:51350
PharmGKB ID(s)PA147357785
General Description of Cytokeratin.
This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45,, 40kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56,, 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research, tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer,, esophageal cancer.

Human, Monkey, Cow, Canine, Rabbit, Mouse, Rat, and Chicken Anti-Cytokeratin Antibody Product Attributes

Species: Human, Monkey, Cow, Canine, Rabbit, Mouse, Rat, and Chicken
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml f), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cytokeratin Antibody Clone: AE-3
Clone AE-3 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Monkey, Cow, Canine, Rabbit, Mouse, Rat, and Chicken Cytokeratin Positive Control Sample: Epithelial cells, Skin or Adenocarcinomas
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human epidermal keratin
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Cytokeratin Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in Cytokeratin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of Cytokeratin could be seen in. We were unable to detect Cytokeratin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Cytokeratin expression as measured by anti-Cytokeratin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + - - + + - + + - - - + + - - - - - + -
KRT76 Variability ++ ++ ++ ++ ++ + ++ ++ ++ + ++ ++ ++ ++ ++ + ++ ++ ++ ++

Limitations and Warranty

enQuire Bio's Cytokeratin, Basic Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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