Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin, HMW Monoclonal Antibody (SPM591).

Human, Mouse, and Rat Anti-Cytokeratin Antibody Clone SPM591

$ 329.00
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SKU: MSM3X-446
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: MSM3X-446-P1
Cytokeratin General Information
Molecular Weight
67kDa (CK1); 58kDa (CK5); 56.5kDa (CK10); 50kDa (CK14)
Chromosomal Location
Curated Database and Bioinformatic Data
Gene SymbolMultiple
Entrez Gene ID3848, 3852, 3858, 3861
Ensemble Gene IDENSG00000167768, ENSG00000186081, ENSG00000186395, ENSG00000186847
RefSeq Protein Accession(s)NP_006112, NP_000415, XP_005257400, NP_000412, NP_000517
RefSeq mRNA Accession(s)NM_006121, NM_000424, NM_000421, XM_005257343NM_000526
RefSeq Genomic Accession(s)NG_008364, NC_000012, NC_018923, NG_008297, NC_000012, NC_018923, NC_018928, NG_033147, NG_008405, NC_000017NG_008624, NC_000017, NC_018928
UniProt ID(s)P04264, P13647, P13645, P02533
UniGene ID(s)P04264, P13647, P13645, P02533
HGNC ID(s)6412, 6442, 6413, 6416
Cosmic ID(s)KRT1, KRT5, KRT10, KRT14
KEGG Gene ID(s)hsa:3848, hsa:3852, hsa:3858, hsa:3861
PharmGKB ID(s)PA30199, PA30230, PA30200, PA30203
General Description of Cytokeratin.
This antibody recognizes CK1, CK5, CK10, CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells, basal cells in the prostate gland, bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors,, neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells, has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.

Human, Mouse, and Rat Anti-Cytokeratin Antibody Product Attributes

Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1.0ug of antibody/ml), Western Blot (0.5-1.0ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cytokeratin Antibody Clone: SPM591
Clone SPM591 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse, and Rat Cytokeratin Positive Control Sample: PC12 cells, Skin, Prostate carcinoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Solubilized keratin extract from human stratum corneum
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Cytokeratin Previously Observed Antibody Staining Patterns

Limitations and Warranty

enQuire Bio's Cytokeratin, Basic Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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