Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-DOG 1 Antibody Clone: DOG1.1
Host and Isotype: Mouse IgG
Recommended Positive Control Sample: Gastrointestinal stromal tumor tissue
Cellular Localization of Antibody DOG1.1 Staining: Cytoplasmic, cell membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide of human DOG1 protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
DOG 1 Information for Pathologists
Summary:
Discovered On GIST 1. Chloride channel protein. Interpretation Membranous and cytoplasmic staining. Uses by pathologistsCommon Uses By Pathologists:
Sensitive and specific marker for GIST (Gut Liver 2011;5:171). May be more sensitive and specific for GIST than CD117 / KIT in cell blocks (Cancer Cytopathol 2011;119:202, Am J Clin Pathol 2011;135:448). Sensitive for CD117 negative GIST tumor (non-GI tumors are usually DOG1+, Am J Surg Pathol 2011;35:1287, Mod Pathol 2011;24:866). Negative staining suggests tumor is not GIST (Cancer Control 2011;18:152). Distinguish gastric plexiform fibromyxoma (alpha smooth muscle actin+, KIT-, DOG1-, CD34-, no KIT / PDGFRA mutations) from GIST (opposite, Am J Surg Pathol 2009;33:1624).Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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