Immunohistochemical staining of DOG 1  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

DOG 1 [DOG1.1] Antibody (cGMP).

$ 135.00$ 515.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD122
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG

Product NumberDescriptionPrice
QIVD122-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD122-0.5ml Size: 0.5 ml, Format: Concentrate $ 275.00
QIVD122-1ml Size: 1 ml, Format: Concentrate $ 515.00
QIVD122-6ml Size: 6 ml, Format: Predilute $ 235.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
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SKU: QIVD122-0.1ml
DOG 1 General Information
Alternate Names
Molecular Weight
114.1 kDa
Chromosomal Location
q13.3 [chr: 11] [chr_start: 70078302] [chr_end: 70189528] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolANO1
Entrez Gene ID55107
RefSeq Protein Accession(s)NP_060513; XP_011543433; XP_006718668
RefSeq mRNA Accession(s)XM_011545121; XM_011545128; NR_030691; XM_011545124; XM_011545131; XM_006718602; XM_011545123; XM_011545126; XM_011545127; XM_006718604; XM_011545129; XM_017017957; NM_018043; XM_006718605; XM_011545125; XM_017017956
RefSeq Genomic Accession(s)NC_000011
UniProt ID(s)Q5XXA6
PharmGKB ID(s)PA164715378
KEGG Gene ID(s)hsa:55107
General Description of DOG 1 .
DOG1 is expressed ubiquitously in gastrointestinal stromal tumors irrespective of c-kit or PDGFR alpha mutation status. Reactivity for DOG1 may aid in the diagnosis of gastrointestinal stromal tumors, including PDGFRA mutants that fail to express c-kit antigen, and lead to appropriate treatment with imatinib mesylate, an inhibitor of the c-kit tyrosine kinase.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment EDTA Buffer pH 8.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-DOG 1 Antibody Clone: DOG1.1
Host and Isotype: Mouse IgG
Recommended Positive Control Sample: Gastrointestinal stromal tumor tissue
Cellular Localization of Antibody DOG1.1 Staining: Cytoplasmic, cell membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide of human DOG1 protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

DOG 1 Information for Pathologists

Summary:

Discovered On GIST 1. Chloride channel protein. Interpretation Membranous and cytoplasmic staining. Uses by pathologists

Common Uses By Pathologists:

Sensitive and specific marker for GIST (Gut Liver 2011;5:171). May be more sensitive and specific for GIST than CD117 / KIT in cell blocks (Cancer Cytopathol 2011;119:202, Am J Clin Pathol 2011;135:448). Sensitive for CD117 negative GIST tumor (non-GI tumors are usually DOG1+, Am J Surg Pathol 2011;35:1287, Mod Pathol 2011;24:866). Negative staining suggests tumor is not GIST (Cancer Control 2011;18:152). Distinguish gastric plexiform fibromyxoma (alpha smooth muscle actin+, KIT-, DOG1-, CD34-, no KIT / PDGFRA mutations) from GIST (opposite, Am J Surg Pathol 2009;33:1624).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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DOG 1 [DOG1.1] Antibody (cGMP).