Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Double Stranded DNA Monoclonal Antibody (AE-2)
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Double Stranded DNA Monoclonal Antibody (AE-2)

Anti-Double Stranded DNA Antibody Clone AE-2

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: MSM2-946
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunocytochemistry Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG3 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: MSM2-946-P1

Human Anti-Double Stranded DNA Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunocytochemistry Immunohistochemistry (IHC).
Application Notes
Flow Cytometry This antibody may be used in flow cytometry with a secondary antibody, although we recommend directly conjugating using an amine-reactive dye and dye removal procedure. Contact us for a protocol. Recommended Concentration for FC is 0.5-1ug of antibody/million cells in 0.1 ml.
ICC/IF Please see our ICC/IF protocol for general recommendations for immunofluorescent staining of cells. Recommended concentration is 1-2ug of antibody/ml. Acetone fixing cells gives optimal staining in our experience with this antibody. Incubate primary 30 minutes at room temp.
Immunohistochemistry (IHC) We recommend this antibody for formain-fixed paraffin embedded (FFPE) tissue sections. See our IHC protocol for general staining protocols and antigen retrieval recommendations. Recommended dilution is 1-2ug of antibody/ml for 30 minutes at RT (dilution factor of 1:100 to 1:200).
Clonality: Monoclonal
Antibody Clone: AE-2
Host and Isotype: Mouse IgG3 kappa
Positive Control Sample for IHC staining of Double Stranded DNA: Tonsil
Cellular Localization of Antibody AE-2 Staining: Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Nuclei of Burkitt's cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Limitations and Warranty

enQuire Bio's Double Stranded DNA Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Double Stranded DNA Antibody Clone AE-2

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