Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Monoclonal Antibody (CDH1/1121+CDH1/1122)

Human Anti-E-Cadherin / CD324 Antibody Clone CDH1/1121 + CDH1/1122

$ 329.00
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SKU: 999-MSM3
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 999-MSM3-P1
E-Cadherin / CD324 General Information
Alternate Names
Cadherin-1, CAM 120/80, Epithelial Cadherin, E-cadherin, CD324
Molecular Weight
120-80kDa (Mature); 135kDa (Precursor)
Chromosomal Location
16q22.1
Curated Database and Bioinformatic Data
Gene SymbolCDH1
Entrez Gene ID999
Ensemble Gene IDENSG00000039068
RefSeq Protein Accession(s)XP_011521790, XP_011521791, NP_001304113, NP_004351, NP_001304115, NP_001304114
RefSeq mRNA Accession(s)XM_011523488, NM_001317184, XM_011523489, NM_001317186, NM_004360, NM_001317185
RefSeq Genomic Accession(s)NG_008021, NC_000016, NC_018927
UniProt ID(s)B3GN61, A0A0U2ZQU7, P12830, Q9UII7
UniGene ID(s)B3GN61, A0A0U2ZQU7, P12830, Q9UII7
HGNC ID(s)1748
Cosmic ID(s)CDH1
KEGG Gene ID(s)hsa:999
PharmGKB ID(s)PA26282
General Description of E-Cadherin / CD324.
Recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure, morphogenesis. The classical cadherins, E-, N-, P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as ? -catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential, poor prognosis in breast cancer, prostate, esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +), lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.

Human Anti-E-Cadherin / CD324 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-E-Cadherin / CD324 Antibody Clone: CDH1/1121 + CDH1/1122
Clone CDH1/1121 + CDH1/1122 Host and Isotype: Mouse IgG
Anti-Human E-Cadherin / CD324 Positive Control Sample: LS174T, Raji, HT29, SK-BR3 cells. Prostate or Colon carcinomas.
Cellular Localization of Antibody Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant fragment (119 Amino acid residues around aa 400-500) of human E-Cadherin protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

E-Cadherin / CD324 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CDH1 antibody staining is expected to be primarily localized to the cell junctions, golgi apparatus and plasma membrane. There is variability in either the signal strength or the localization of signal in plasma membrane from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in E-Cadherin / CD324 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the tubules in kidney, epidermal cells in the skin, exocrine glandular cells in the pancreas, glandular cells in the appendix, breast, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, keratinocytes in skin, Langerhans in skin, melanocytes in skin, pneumocytes in lung, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in cells in the tubules in kidney, epidermal cells in the skin, exocrine glandular cells in the pancreas, glandular cells in the appendix, breast, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, keratinocytes in skin, Langerhans in skin, melanocytes in skin, pneumocytes in lung, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. Low, but measureable presence of E-Cadherin / CD324 could be seen inmacrophages in lung. We were unable to detect E-Cadherin / CD324 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of E-Cadherin / CD324 expression as measured by anti-E-Cadherin / CD324 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ ++ +++ +++ +++ - ++ ++ ++ - ++ +++ +++ +++ - ++ +++ ++ +++ +++
CDH1 Variability ++ ++ ++ + + + ++ ++ ++ + ++ ++ + + ++ ++ ++ ++ + ++

Limitations and Warranty

enQuire Bio's E-Cadherin / CD324 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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