enQuire Bio » Products » Antibody » E-Cadherin [SPM471] Antibody (cGMP).
Immunohistochemical staining of E-Cadherin of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

E-Cadherin [SPM471] Antibody (cGMP).

$ 135.00 – $ 675.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD123
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG 2b/k

Product NumberDescriptionPrice
QIVD123-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD123-0.5ml Size: 0.5 ml, Format: Concentrate $ 415.00
QIVD123-1ml Size: 1 ml, Format: Concentrate $ 675.00
QIVD123-6ml Size: 6 ml, Format: Predilute $ 295.00
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In Stock
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
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SKU: QIVD123-0.1ml
E-Cadherin General Information
Alternate Names
Molecular Weight
97.5 kDa
Chromosomal Location
q22.1 [chr: 16] [chr_start: 68737292] [chr_end: 68835541] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolCDH1
Entrez Gene ID999
RefSeq Protein Accession(s)NP_004351; NP_001304115; NP_001304113; NP_001304114
RefSeq mRNA Accession(s); NM_001317184; NM_001317186; NM_004360; NM_001317185
RefSeq Genomic Accession(s)NG_008021; NC_000016
UniProt ID(s)P12830
PharmGKB ID(s)PA26282
KEGG Gene ID(s)hsa:999
Associated Diseases (KEGG IDs)Hereditary diffuse gastric cancer (HDGC) [MIM:137215]: A cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. {ECO:0000269|PubMed:10319582, ECO:0000269|PubMed:12216071}. Disease susceptibility is associated with variations affecting the gene represented in this entry. Heterozygous CDH1 germline mutations are responsible for familial cases of diffuse gastric cancer. Somatic mutations has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.; Endometrial cancer (ENDMC) [MIM:608089]: A malignancy of endometrium, the mucous lining of the uterus. Most endometrial cancers are adenocarcinomas, cancers that begin in cells that make and release mucus and other fluids. Disease susceptibility is associated with variations affecting the gene represented in this entry.; Ovarian cancer (OC) [MIM:167000]: The term ovarian cancer defines malignancies originating from ovarian tissue. Although many histologic types of ovarian tumors have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease. {ECO:0000269|PubMed:8075649}. Disease susceptibility is associated with variations affecting the gene represented in this entry.; Breast cancer, lobular (LBC) [MIM:137215]: A type of breast cancer that begins in the milk-producing glands (lobules) of the breast. {ECO:0000269|PubMed:17660459}. The gene represented in this entry may be involved in disease pathogenesis.; Blepharocheilodontic syndrome 1 (BCDS1) [MIM:119580]: A form of blepharocheilodontic syndrome, a rare autosomal dominant disorder. It is characterized by lower eyelid ectropion, upper eyelid distichiasis, euryblepharon, bilateral cleft lip and palate, and features of ectodermal dysplasia, including hair anomalies, conical teeth and tooth agenesis. An additional rare manifestation is imperforate anus. There is considerable phenotypic variability among affected individuals. {ECO:0000269|PubMed:28301459}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of E-Cadherin .
E-cadherin (uvomorulin, cell-CAM120/80) is a calcium dependent cell adhesion molecule expressed predominately in epithelial tissues. It plays an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Numerous studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:50
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-E-Cadherin Antibody Clone: SPM471
Host and Isotype: Mouse IgG 2b/k
Recommended Positive Control Sample: Breast carcinoma, Colon carcinoma, Appendix
Cellular Localization of Antibody SPM471 Staining: Cell Membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Recombinant protein encoding human Cadherin-E 600-707aa
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

E-Cadherin Information for Pathologists

Summary:

Epithelial calcium-dependent cell adhesion protein. Calcium dependent transmembrane epithelial protein that promotes intercellular adhesion. Its loss is associated with invasive carcinoma. Tumor suppressor gene CDH1 is at 16q22.1. Abnormal function in carcinomas may facilitate cellular detachment leading to metastasis.

Common Uses By Pathologists:

Distinguish LCIS from DCIS in indeterminate cases (Am J Surg Pathol 2001;25:229). Marker of immature erythroid precursors (Am J Clin Pathol 2014;141:656). Microscopic (histologic) images Images hosted on other servers:. Missing Image.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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E-Cadherin [SPM471] Antibody (cGMP).