Human Anti-EGFR Antibody Product Attributes
Tested Applications: Western Blot. Additionally, this antibody has been validated by MS-QAVAâ¢. Specificity has been quantitatively determined via Mass Spec.
Anti-EGFR Antibody Clone: H9B4
Clone H9B4 Host and Isotype: Mouse IgG1
Anti-Human EGFR Positive Control Sample: A431 cells, Breast or Bladder cancer.
Cellular Localization of Antibody
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Purified EGFR from A431 cells.
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
EGFR Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:Variations in EGFR antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in trophoblastic cells in the placenta. More moderate antibody staining intensity was present in trophoblastic cells in the placenta. Low, but measureable presence of EGFR could be seen inhepatocytes in liver, Leydig cells in the testis, melanocytes in skin, myocytes in skeletal muscle, squamous epithelial cells in the tonsil and vagina and urothelial cells in the urinary bladder. We were unable to detect EGFR in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Type:Tissues from cancer patients, for instance, have their own distinct pattern of EGFR expression as measured by anti-EGFR antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Limitations and Warranty
This product is for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.