Formalin-fixed, paraffin-embedded human Placenta stained with EGFR Monoclonal Antibody (SPM622).
Formalin-fixed, paraffin-embedded human Placenta stained with EGFR Monoclonal Antibody (SPM622).

Anti-EGFR Antibody Clone SPM622

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 1956-MSM4X
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
1956-MSM4X-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
Flat Rate Shipping Anywhere in the US: $45
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SKU: 1956-MSM4X-P1
EGFR General Information
Alternate Names
EGFR, ErbB-1, HER1, ErbB1
Molecular Weight
~170kDa (wild type), ~145kDa (vIII variant)
Chromosomal Location
7p11.2
Curated Database and Bioinformatic Data
Gene SymbolEGFR
Entrez Gene ID1956
Ensemble Gene IDENSG00000146648
RefSeq Protein Accession(s)NP_001333827, NP_958439, NP_001333829, NP_958440, NP_001333826, NP_958441, NP_005219, NP_001333870, NP_001333828
RefSeq mRNA Accession(s)NM_201282, NM_001346897, NM_201283, NM_001346898, NM_001346941 NM_201284, NM_001346900, NM_001346899, NM_005228
RefSeq Genomic Accession(s)NC_018918, NG_007726, NC_000007
UniProt ID(s)Q504U8, B7Z2I3, E9PFD7, P00533, F2YGG7, E7BSV0
UniGene ID(s)Q504U8, B7Z2I3, E9PFD7, P00533, F2YGG7, E7BSV0
HGNC ID(s)3236
Cosmic ID(s)EGFR
KEGG Gene ID(s)hsa:1956
PharmGKB ID(s)PA7360
General Description of EGFR.
This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin, epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary,, endometrium. It is predominantly present in squamous cell carcinomas.

Human Anti-EGFR Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (2-4ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-EGFR Antibody Clone: SPM622
Clone SPM622 Host and Isotype: Mouse IgG1 kappa
Anti-Human EGFR Positive Control Sample: A431 cells. Placenta, Breast, Colon or Bladder cancer
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human EGFR purified from A431 cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

EGFR Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-EGFR antibody staining is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in EGFR antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in trophoblastic cells in the placenta. More moderate antibody staining intensity was present in trophoblastic cells in the placenta. Low, but measureable presence of EGFR could be seen inhepatocytes in liver, Leydig cells in the testis, melanocytes in skin, myocytes in skeletal muscle, squamous epithelial cells in the tonsil and vagina and urothelial cells in the urinary bladder. We were unable to detect EGFR in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of EGFR expression as measured by anti-EGFR antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - + - - - - - - - - - - - - - -
EGFR Variability + + + + + ++ + ++ + + + + + + + + + + + +

Limitations and Warranty

enQuire Bio's EGFR Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-EGFR Antibody Clone SPM622

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