Human and Rat (-) Anti-Epcam / CD326 Antibody Product Attributes
Species: Human and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1.0ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 min at RT)
Anti-Epcam / CD326 Antibody Clone: SPM528
Clone SPM528 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Rat (-) Epcam / CD326 Positive Control Sample: HT29 cells or breast tumor
Cellular Localization of Antibody
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Neuraminidase treated GLS-1 human small cell lung carcinoma cells
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
Epcam / CD326 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:Anti-EPCAM antibody staining is expected to be primarily localized to the plasma membrane.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of Epcam / CD326 expression as measured by anti-Epcam / CD326 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Limitations and Warranty
enQuire Bio's Epcam / CD326 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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