Human Anti-ER-beta1 Antibody Product Attributes
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Anti-ER-beta1 Antibody Clone: ESR2/686
Clone ESR2/686 Host and Isotype: Mouse IgG2a
Anti-Human ER-beta1 Positive Control Sample: MCF-7 Cells. Ovarian, Breast, Bladder, Gastric or Salivary carcinoma
Cellular Localization of Antibody Predominantly nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: C-terminus fragment of recombinant human estrogen receptor beta protein
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
ER-beta1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-ESR2 antibody staining is expected to be primarily localized to the nucleoplasm.
Limitations and Warranty
enQuire Bio's ER-beta1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.