Immunohistochemical staining of ERCC1  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

ERCC1 [8F1] Antibody (cGMP).

$ 135.00$ 775.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD128
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG2b

Product NumberDescriptionPrice
QIVD128-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD128-0.5ml Size: 0.5 ml, Format: Concentrate $ 465.00
QIVD128-1ml Size: 1 ml, Format: Concentrate $ 775.00
QIVD128-6ml Size: 6 ml, Format: Predilute $ 445.00
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Datasheets and Documentation
Product Datasheet
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SKU: QIVD128-0.1ml
ERCC1 General Information
Alternate Names
Molecular Weight
32.6 kDa
Chromosomal Location
q13.32 [chr: 19] [chr_start: 45407333] [chr_end: 45478828] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolERCC1
Entrez Gene ID2067
RefSeq Protein Accession(s)XP_005258693; NP_001974; XP_011524912; XP_016881954; XP_005258691; XP_005258692; XP_016881951; XP_016881952; XP_016881950; XP_016881948; NP_001159521; XP_016881949; XP_016881953; NP_973730; XP_016881955
RefSeq mRNA Accession(s); NM_001369413; NM_001369414; NM_001369418; NM_001983; NM_001369408; NM_001369411; NM_001369412; NM_001369417; NM_001369415; NM_001166049; NM_001369410; NM_001369416; NM_001369409; NM_001369419; NM_202001
RefSeq Genomic Accession(s)NC_000019; NG_015839
UniProt ID(s)P07992
PharmGKB ID(s)PA155
KEGG Gene ID(s)hsa:2067
Associated Diseases (KEGG IDs)Cerebro-oculo-facio-skeletal syndrome 4 (COFS4) [MIM:610758]: A disorder of prenatal onset characterized by microcephaly, congenital cataracts, facial dysmorphism, neurogenic arthrogryposis, growth failure and severe psychomotor retardation. COFS is considered to be part of the nucleotide-excision repair disorders spectrum that include also xeroderma pigmentosum, trichothiodystrophy and Cockayne syndrome. {ECO:0000269|PubMed:17273966, ECO:0000269|PubMed:23623389}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of ERCC1 .
This antibody reacts with a 33-36 kDa protein known as ERCC1 (excision repair cross complementing) polypeptide. ERCC1 is required for nucleotide excision repair of damaged DNA and is homologous to RAD10. In mammalian cells, XPG cleaves 3’ of the DNA lesion while ERCC1-XPF complex makes the 5’ incision.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:100-1:200
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-ERCC1 Antibody Clone: 8F1
Host and Isotype: Mouse IgG2b
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody 8F1 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with full length recombinant human ERCC1 protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

ERCC1 Information for Pathologists

Summary:

Excision Repair Cross-Complementation group 1. DNA repair gene. Genetic polymorphisms may affect patient response to platinum-based chemotherapy in non-small cell lung carcinoma (Clin Lung Cancer 2009;10:118, Clin Cancer Res 2004;10:4939, Eur J Cardiothorac Surg 2008;33:805). Diagrams / tables Missing Image.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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ERCC1 [8F1] Antibody (cGMP).