C57Bl/6 bone marrow cells were stained with FITC Mouse Anti-CD11b  and 0.5 ug APC Mouse Anti-F4/80 Antigen (QAB68) (right panel) or 0.5 ug APC Rat IgG2a isotype control (left panel).
C57Bl/6 bone marrow cells were stained with FITC Mouse Anti-CD11b  and 0.5 ug APC Mouse Anti-F4/80 Antigen (QAB68) (right panel) or 0.5 ug APC Rat IgG2a isotype control (left panel).Human PBMCs were stained with 5 uL  .C57Bl/6 bone marrow cells were stained with APC Mouse Anti-CD11b  and 0.25 ug FITC Mouse Anti-F4/80 Antigen  (right panel) or 0.25 ug FITC Rat IgG2b isotype control (left panel).C57Bl/6 bone marrow cells were stained with APC Mouse Anti-CD11b  and 0.25 ug PerCP-Cy5.5 Mouse Anti-F4/80 Antigen  .C57Bl/6 bone marrow cells were stained with APC Mouse Anti-CD11b  and 0.25 ug PE Mouse Anti-F4/80 Antigen  (right panel) or 0.25 ug PE Rat IgG2b isotype control (left panel).C57Bl/6 bone marrow cells were stained with APC Mouse Anti-CD11b  and 0.25 ug PE-Cy7 Mouse Anti-F4/80 Antigen .C57Bl/6 bone marrow cells were stained with FITC Mouse Anti-CD11b  and 0.25 ug Qfluor™ 710 Mouse Anti-F4/80 Antigen (QAB68) (right panel) or 0.25 ug Qfluor™ 710 Rat IgG2b isotype control (left panel).C57Bl/6 bone marrow cells were stained with V450 Mouse Anti-CD11b  and 0.25 ug V450 Mouse Anti-F4/80 Antigen  (right panel) or 0.25 ug V450 Rat IgG2b isotype control (left panel).

Anti-F4/80 Antigen Antibody [BM8.1]

$ 48.00$ 358.00
Please Select Product Options Below To View The Catalog Number.

SKU: QAB68
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: Biotin ;Unconjugated ;APC ;APC-Cy7 ;FITC ;PerCP-Cy5.5 ;PE ;PE-Cy7 ;Qfluor 710 ;V450
Isotype: Rat IgG2b kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB68-B-25ug
F4/80 General Information
Alternate Names
EGF-like module-containing mucin-like hormone receptor-like 1, EFR1
Curated Database and Bioinformatic Data
Gene SymbolAdgre1
Entrez Gene ID13733
Ensemble Gene IDENSMUSG00000004730
RefSeq Protein Accession(s)NP_034260, XP_006523665, XP_006523664
RefSeq mRNA Accession(s)XM_006523601, NM_010130, XM_006523602
RefSeq Genomic Accession(s)NC_000083
UniProt ID(s)Q61549, Q3U9R0
UniGene ID(s)Q61549, Q3U9R0
Cosmic ID(s)Adgre1
KEGG Gene ID(s)mmu:13733
General Description of F4/80.
The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

Mouse Anti-F4/80 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-F4/80 Antibody Clone: BM8.1
Clone BM8.1 Host and Isotype: Rat IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

F4/80 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of F4/80 expression as measured by anti-F4/80 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - - - - - - - - - - - -
ADGRE1 Variability + ++ + + ++ ++ + + + + + ++ + ++ + + + + + +

Selected References

Ioannou M, Alissafi T, Boon L, Boumpas D, and Verginis P. 2013. J. Immunol. 190: 2631-2640. (Flow Cytometry)Papadopoulos G, Weinberg EO, Massari P, Gibson FC, Wetzler LM, Morgan EF, and Genco CA. 2013. J. Immunol. 190: 1148-1157. Chen Q and Snapper CM. 2013. J. Immunol. 190: 1048-1055. Rankin AL, Mumm JB, Murphy E, Turner S, Yu N, McClanahan TK, Bourne PA, Pierce RH, Kastelein R and Pflanz S. 2010. J. Immunol. 184(3): 1526-1535. (Immunohistochemistry - paraffin-embedded tissue)Geutskens SB, Otonkoski T, Pulkkinen MA, Drexhage HA and Leenen PJ. 2005. J. Leukoc. Biol. 78(4): 845-52 (Immunohistochemistry - frozen tissue)

Limitations and Warranty

enQuire Bio's Mouse Anti-F4/80 Antigen Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-F4/80 Antigen Antibody [BM8.1]

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