Mouse Anti-F4/80 Antibody Product Attributes
Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-F4/80 Antibody Clone: BM8.1
Clone BM8.1 Host and Isotype: Rat IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
F4/80 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of F4/80 expression as measured by anti-F4/80 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
ADGRE1 Variability | + | ++ | + | + | ++ | ++ | + | + | + | + | + | ++ | + | ++ | + | + | + | + | + | + |
Selected References
Ioannou M, Alissafi T, Boon L, Boumpas D, and Verginis P. 2013. J. Immunol. 190: 2631-2640. (Flow Cytometry)Papadopoulos G, Weinberg EO, Massari P, Gibson FC, Wetzler LM, Morgan EF, and Genco CA. 2013. J. Immunol. 190: 1148-1157. Chen Q and Snapper CM. 2013. J. Immunol. 190: 1048-1055. Rankin AL, Mumm JB, Murphy E, Turner S, Yu N, McClanahan TK, Bourne PA, Pierce RH, Kastelein R and Pflanz S. 2010. J. Immunol. 184(3): 1526-1535. (Immunohistochemistry - paraffin-embedded tissue)Geutskens SB, Otonkoski T, Pulkkinen MA, Drexhage HA and Leenen PJ. 2005. J. Leukoc. Biol. 78(4): 845-52 (Immunohistochemistry - frozen tissue)
Limitations and Warranty
enQuire Bio's Mouse Anti-F4/80 Antigen Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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