Human Anti-Fascin-1 Antibody Product Attributes
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1.0ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Anti-Fascin-1 Antibody Clone: FSCN1/417
Clone FSCN1/417 Host and Isotype: Mouse IgG2a kappa
Anti-Human Fascin-1 Positive Control Sample: HeLa, MCF-7, PC-3 or BEWO cells. HodgkinÂs lymphoma, Ovarian or Testicular Carcinoma.
Cellular Localization of Antibody
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Full length recombinant human FSCN1 protein
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
Fascin-1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:Anti-FSCN1 antibody staining is expected to be primarily localized to the cytosol and plasma membrane.
Observed Antibody Staining Data By Tissue Type:Variations in Fascin-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the glomeruli in kidney, cells in the molecular layer in cerebellum, cells in the seminiferous ducts in testis, glandular cells in the breast, glial cells in the caudate nucleus and cerebral cortex, Langerhans in skin and squamous epithelial cells in the tonsil. More moderate antibody staining intensity was present in cells in the glomeruli in kidney, cells in the molecular layer in cerebellum, cells in the seminiferous ducts in testis, glandular cells in the breast, glial cells in the caudate nucleus and cerebral cortex, Langerhans in skin and squamous epithelial cells in the tonsil. Low, but measureable presence of Fascin-1 could be seen indecidual cells in the placenta, germinal center cells in the lymph node and tonsil, hematopoietic cells in the bone marrow, keratinocytes in skin, lymphoid tissue in appendix, neuronal cells in the caudate nucleus, neuropil in cerebral cortex, non-germinal center cells in the tonsil, pneumocytes in lung, squamous epithelial cells in the cervix and uterine, esophagus, oral mucosa and vagina. We were unable to detect Fascin-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of Fascin-1 expression as measured by anti-Fascin-1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Limitations and Warranty
enQuire Bio's Fascin-1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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