Immunohistochemical staining of Fascin-1  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Fascin-1 [55k-2] Antibody (cGMP).

$ 135.00$ 625.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD141
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG2a, kappa

Product NumberDescriptionPrice
QIVD141-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD141-0.5ml Size: 0.5 ml, Format: Concentrate $ 445.00
QIVD141-1ml Size: 1 ml, Format: Concentrate $ 625.00
QIVD141-6ml Size: 6 ml, Format: Predilute $ 245.00
QIVD141-25ml Size: 25 ml, Format: Predilute $ 625.00
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In Stock
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
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SKU: QIVD141-0.1ml
Fascin-1 General Information
Alternate Names
Molecular Weight
54.5 kDa
Chromosomal Location
p22.1 [chr: 7] [chr_start: 5592816] [chr_end: 5606655] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolFSCN1
Entrez Gene ID6624
RefSeq Protein Accession(s)NP_003079
RefSeq mRNA Accession(s); NM_003088
RefSeq Genomic Accession(s)NC_000007; NG_030004
UniProt ID(s)Q16658
PharmGKB ID(s)PA128394534
KEGG Gene ID(s)hsa:6624
General Description of Fascin-1 .
Recognizes a protein of 55kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin s disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Fascin-1 Antibody Clone: 55k-2
Host and Isotype: Mouse IgG2a, kappa
Recommended Positive Control Sample: Hodgkin s lymphoma, Ovary
Cellular Localization of Antibody 55k-2 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Full length recombinant human FSCN1 protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Fascin-1 Information for Pathologists

Summary:

Fascin-1 is an actin bundling protein with important role in cell motility and adhesion. High expression in tumors is generally a poor prognostic marker. Fascin-1 is present in specialized cells with extensive surfaces or migratory potential, such as neurons, glia, dendritic cells, macrophages, skeletal and smooth muscle, endothelial cells but is generally absent in normal epithelial cells.

Common Uses By Pathologists:

Facin-1 staining is generally observed in carcinoma of biliary tract, breast, colon, lung, ovary, pancreas, and skin. Follicular dendritic cell tumors, Hodgkin lymphoma-classic subtype (highly sensitive), interdigitating dendritic cell tumors, Langerhan cell histiocytosis, and urothelial carcinoma (noninvasive papillary or invasive) also express Fascin-1.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Fascin-1 [55k-2] Antibody (cGMP).