Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with FOXA1 Monoclonal Antibody (FOXA1/1514).
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with FOXA1 Monoclonal Antibody (FOXA1/1514).

Anti-FOXA1 / HNF3A Antibody Clone FOXA1/1514

$ 118.00$ 418.00
Please Select Product Options Below To View The Catalog Number.

SKU: 3169-MSM4
Species: Human
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 3169-MSM4-P0
FOXA1 / HNF3A General Information
Alternate Names
Forkhead box protein A1, FOXA1, hepatocyte nuclear factor 3-alpha, HNF-3A, FOXA1
Molecular Weight
79kDa
Chromosomal Location
14q21.1
Curated Database and Bioinformatic Data
Gene SymbolFOXA1
Entrez Gene ID3169
Ensemble Gene IDENSG00000129514
RefSeq Protein Accession(s)XP_016876735, NP_004487
RefSeq mRNA Accession(s)NM_004496
RefSeq Genomic Accession(s)NG_033028, NC_018925, NC_000014
UniProt ID(s)P55317
UniGene ID(s)P55317
HGNC ID(s)5021
Cosmic ID(s)FOXA1
KEGG Gene ID(s)hsa:3169
PharmGKB ID(s)PA201090
General Description of FOXA1 / HNF3A.
The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (? and ?), HNF-3 (?, ? and ?), HNF-4 (? and ?), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.

Human Anti-FOXA1 / HNF3A Antibody Product Attributes

Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-FOXA1 / HNF3A Antibody Clone: FOXA1/1514
Clone FOXA1/1514 Host and Isotype: Mouse IgG2b kappa
Anti-Human FOXA1 / HNF3A Positive Control Sample: HepG-2 or MCF-7 cells. Liver, Pancreas, Bladder, Prostate, Colon and Lung and Breast.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human FOXA1 protein fragment (aa467-614)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

FOXA1 / HNF3A Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-FOXA1 antibody staining is expected to be primarily localized to the nucleoli fibrillar center and nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in FOXA1 / HNF3A antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the prostate. More moderate antibody staining intensity was present in glandular cells in the prostate. Low, but measureable presence of FOXA1 / HNF3A could be seen inglandular cells in the appendix, breast, colon, duodenum, gallbladder, rectum and small intestine, squamous epithelial cells in the esophagus and oral mucosa and glandular cells in the appendix, breast, colon, duodenum, gallbladder and rectum and small intestine. We were unable to detect FOXA1 / HNF3A in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of FOXA1 / HNF3A expression as measured by anti-FOXA1 / HNF3A antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ ++ - - - - - - - - - - - ++ - - - - - ++
FOXA1 Variability + ++ ++ ++ ++ + ++ + ++ + + + ++ ++ + + ++ + + +++

Limitations and Warranty

enQuire Bio's FOXA1 / HNF3A Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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