Mouse Anti-FOXP3 Antibody Product Attributes
Cross Reactivity: rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-FOXP3 Antibody Clone: 3G3
Clone 3G3 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.05% BSA, 0.05% NaN3, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
FOXP3 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:Staining with anti-FOXP3 antibody reveals FOXP3 expression is expected to be primarily localized to the nucleoplasm.
Observed Antibody Staining Data By Tissue Type:Variations in FOXP3 / Scurfin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of FOXP3 / Scurfin could be seen inLeydig cells in the testis and macrophages in lung. We were unable to detect FOXP3 / Scurfin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Ramos RN, Oliveira CE, Gasparoto TH, et al. 2012. Carcinogenesis. 33: 902-909. (Flow cytometry) Klein M, Vaeth M, Scheel T, Grabbe S, Baumgrass R, Berberich-Siebelt F, Bopp T, Schmitt E, and Becker C. 2012. J. Immunol. 188: 1091-1097. (Flow cytometry)Ansari AA, Reimann KA, Mayne AE, Takahashi Y, Stephenson ST, Wang R, Wang X, Li J, Price AA, Little DM, Zaidi M, Lyles R, and Villinger F. 2011. J. Immunol. 186: 1044-1059. (Flow cytometry - Rhesus macaque)Nagar M, Vernitsky H, Cohen Y, Dominissini D, Berkun Y, Rechavi G, Amariglio N, and Goldstein I. 2008. Int. Immunol. 20: 1041-1055. (Flow cytometry)Hombach AA, Kofler D, Hombach A, Rappl G, and Abken H. 2007. J. Immunol. 179: 7924-7931. (Flow cytometry).Gavin MA, Torgerson TR, Houston E, deRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, and Rudensky AY. 2006. Proc Natl Acad Sci USA. 103(17): 6659-6664. (Flow cytometry)
Limitations and Warranty
enQuire Bio's Human Anti-/ Mouse Foxp3 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.