C57Bl/6 splenocytes were stained with FITC Mouse Anti-CD4 , followed by intracellular staining with 0.125 ug APC Mouse Anti-Foxp3 (QAB71) (right panel) or 0.125 ug APC Mouse IgG1 isotype control (left panel).

Human Anti-/ Mouse Foxp3 Antibody [3G3]

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Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: PE ;APC ;FITC ;PerCP-Cy5.5 ;PE-Cy7
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB71-PE-25ug
FOXP3 General Information
Alternate Names
FOXP3, forkhead box P3, scurfin
Curated Database and Bioinformatic Data
Gene SymbolFoxp3
Entrez Gene ID50943, 20371
Ensemble Gene IDENSG00000049768, ENSMUSG00000039521
RefSeq Protein Accession(s)XP_016885054, XP_016885056, XP_006724596, XP_016885055, NP_001107849, XP_011542218, NP_054728, NP_473380, NP_001186277, NP_001186276
RefSeq mRNA Accession(s)XM_011543916, XM_017029566, XM_006724533, XM_017029567, NM_001114377, NM_014009NM_054039, NM_001199347, NM_001199348
RefSeq Genomic Accession(s)NG_007392, NC_018934, NC_000023, NC_000086
UniProt ID(s)Q9BZS1, B7ZLG1, Q53Z59, Q99JB6
UniGene ID(s)Q9BZS1, B7ZLG1, Q53Z59, Q99JB6
HGNC ID(s)6106
Cosmic ID(s)FOXP3, Foxp3
KEGG Gene ID(s)hsa:50943, mmu:20371
PharmGKB ID(s)PA201094
General Description of FOXP3.
The 3G3 antibody reacts with mouse Foxp3, a 50-55 kDa transcription factor which is a central regulator of T cell activity and is critical for the development and function of regulatory T cells (Tregs). Foxp3 is expressed at constitutively high levels in Treg cells, which are further identified as being CD4+ CD25+. In resting conventional T cells (CD4+ CD25-) Foxp3 expression is restricted, and upon TCR activation is expressed only transiently and in a small proportion of cells. However, the growth factor TGF-beta has been shown to induce expression of Foxp3 in nave T cells, driving their development into Foxp3+ Tregs, which are called induced or adaptive Tregs. These cells are phenotypically similar to so-called natural Tregs (CD4+ CD25high Foxp3+) which originate in the thymus and comprise the majority of Treg cells. Tregs are critical for maintaining peripheral tolerance and are implicated in the development of autoimmunity.It is important to review the literature in choosing an antibody for the Foxp3 antigen in flow cytometry, as the potential for high background or non-specific staining may be observed. The 3G3 antibody may be used for intracellular detection of Foxp3 in cells from mouse and Rhesus macaque.

Mouse Anti-FOXP3 Antibody Product Attributes

Species: Mouse
Cross Reactivity: rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-FOXP3 Antibody Clone: 3G3
Clone 3G3 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.05% BSA, 0.05% NaN3, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

FOXP3 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-FOXP3 antibody reveals FOXP3 expression is expected to be primarily localized to the nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in FOXP3 / Scurfin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of FOXP3 / Scurfin could be seen inLeydig cells in the testis and macrophages in lung. We were unable to detect FOXP3 / Scurfin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Ramos RN, Oliveira CE, Gasparoto TH, et al. 2012. Carcinogenesis. 33: 902-909. (Flow cytometry) Klein M, Vaeth M, Scheel T, Grabbe S, Baumgrass R, Berberich-Siebelt F, Bopp T, Schmitt E, and Becker C. 2012. J. Immunol. 188: 1091-1097. (Flow cytometry)Ansari AA, Reimann KA, Mayne AE, Takahashi Y, Stephenson ST, Wang R, Wang X, Li J, Price AA, Little DM, Zaidi M, Lyles R, and Villinger F. 2011. J. Immunol. 186: 1044-1059. (Flow cytometry - Rhesus macaque)Nagar M, Vernitsky H, Cohen Y, Dominissini D, Berkun Y, Rechavi G, Amariglio N, and Goldstein I. 2008. Int. Immunol. 20: 1041-1055. (Flow cytometry)Hombach AA, Kofler D, Hombach A, Rappl G, and Abken H. 2007. J. Immunol. 179: 7924-7931. (Flow cytometry).Gavin MA, Torgerson TR, Houston E, deRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, and Rudensky AY. 2006. Proc Natl Acad Sci USA. 103(17): 6659-6664. (Flow cytometry)

Limitations and Warranty

enQuire Bio's Human Anti-/ Mouse Foxp3 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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