enQuire-Bio-QAB27-APC-100ug-anti-FOXP3-Scurfin-antibody-10
C57Bl/6 splenocytes were stained with APC Mouse Anti-CD4 , followed by intracellular staining with 0.25 ug PE Mouse Anti-Foxp3 (QAB27) (right panel) or 0.25 ug PE Rat IgG2b isotype control (left panel).

Anti-Foxp3 Antibody [MF23]

$ 139.00$ 319.00
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SKU: QAB27
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: APC ;PE
Isotype: Rat IgG2b
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB27-APC-25ug
FOXP3 / Scurfin General Information
Alternate Names
FOXP3, Scurfin, AIID, DIETER, XPID, JM2, IPEX, PIDX
Curated Database and Bioinformatic Data
Gene SymbolFoxp3
Entrez Gene ID20371
Ensemble Gene IDENSMUSG00000039521
RefSeq Protein Accession(s)NP_473380, NP_001186277, NP_001186276
RefSeq mRNA Accession(s)NM_054039, NM_001199347, NM_001199348
RefSeq Genomic Accession(s)NC_000086
UniProt ID(s)Q53Z59, Q99JB6
UniGene ID(s)Q53Z59, Q99JB6
Cosmic ID(s)Foxp3
KEGG Gene ID(s)mmu:20371
General Description of FOXP3 / Scurfin.
The MF23 antibody reacts with mouse Foxp3, a 50-55 kDa transcription factor which is a central regulator of T cell activity and is critical for the development and function of regulatory T cells (Tregs). Foxp3 is a member of the forkhead box or winged helix family of transcription factors and is expressed at constitutively high levels in Treg cells. Forced expression of Foxp3 in conventional T cells results in the upregulation of Treg associated molecules such as CD25, CTLA-4, and GITR and is sufficient to impart suppressive functional activity to these cells.

Mouse Anti-FOXP3 / Scurfin Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-FOXP3 / Scurfin Antibody Clone: MF23
Clone MF23 Host and Isotype: Rat IgG2b
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

FOXP3 / Scurfin Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-FOXP3 / Scurfin antibody reveals FOXP3 / Scurfin expression is expected to be primarily localized to the nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in FOXP3 / Scurfin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of FOXP3 / Scurfin could be seen inLeydig cells in the testis and macrophages in lung. We were unable to detect FOXP3 / Scurfin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Ramos RN, Oliveira CE, Gasparoto TH, et al. 2012. Carcinogenesis. 33: 902-909. (Flow cytometry) Klein M, Vaeth M, Scheel T, Grabbe S, Baumgrass R, Berberich-Siebelt F, Bopp T, Schmitt E, and Becker C. 2012. J. Immunol. 188: 1091-1097. (Flow cytometry)Ansari AA, Reimann KA, Mayne AE, Takahashi Y, Stephenson ST, Wang R, Wang X, Li J, Price AA, Little DM, Zaidi M, Lyles R, and Villinger F. 2011. J. Immunol. 186: 1044-1059. (Flow cytometry - Rhesus macaque)Nagar M, Vernitsky H, Cohen Y, Dominissini D, Berkun Y, Rechavi G, Amariglio N, and Goldstein I. 2008. Int. Immunol. 20: 1041-1055. (Flow cytometry)Hombach AA, Kofler D, Hombach A, Rappl G, and Abken H. 2007. J. Immunol. 179: 7924-7931. (Flow cytometry).Gavin MA, Torgerson TR, Houston E, deRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, and Rudensky AY. (Flow cytometry)

Limitations and Warranty

enQuire Bio's Mouse Anti-Foxp3 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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