Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
|Recommended Dilution (Conc)||1:50-1:100|
|Pretreatment||Citrate Buffer pH 6.0|
|Incubation Parameters||30 min at Room Temperature|
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Anti-Galectin-3 Antibody Clone: H-5
Host and Isotype: Mouse IgG2a, kappa
Recommended Positive Control Sample: Colon Carcinoma, Prostate
Cellular Localization of Antibody H-5 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were immunized with prokaryotic recombinant fusion protein corresponding to the full-length galectin-3 molecule.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Galectin-3 Information for Pathologists
Summary:Member of carbohydrate-binding protein family known as lectins. One of 14 galectins, which function as cell receptors for N-acetyl-lactosamine moieties present on most extracellular matrix components. Also member of the beta-galactoside-binding protein family that plays an important role in cell - cell adhesion, cell - matrix interactions, macrophage activation, angiogenesis, metastasis, apoptosis. Uses by pathologists In one study, Gal-3+ with Ki67 > 6% was associated with parathyroid carcinomas vs. adenomas (Hum Pathol 2005;36:908).
Common Uses By Pathologists:In one study, Gal-3+ with Ki67 > 6% was associated with parathyroid carcinomas vs. adenomas (Hum Pathol 2005;36:908). Positive staining - normal Endothelial cells, peripheral nerve, folliculostellate cells of adenohypophysis. Positive staining - disease Tumors of thyroid, head and neck, liver, colon, prolactinomas; parathyroid carcinoma; rarely in reactive of hyperplastic parathyroid lesions.
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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