Immunohistochemical staining of GATA3  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

GATA3 [L50-823] Antibody (cGMP).

$ 175.00$ 1,125.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD131
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1

Product NumberDescriptionPrice
QIVD131-0.1ml Size: 0.1 ml, Format: Concentrate $ 175.00
QIVD131-0.5ml Size: 0.5 ml, Format: Concentrate $ 675.00
QIVD131-1ml Size: 1.0 ml, Format: Concentrate $ 1,125.00
QIVD131-6ml Size: 6 ml, Format: Predilute $ 225.00
QIVD131-25ml Size: 25 ml, Format: Predilute $ 875.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
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SKU: QIVD131-0.1ml
GATA3 General Information
Alternate Names
Molecular Weight
47.9 kDa
Chromosomal Location
p14 [chr: 10] [chr_start: 8045378] [chr_end: 8075198] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolGATA3
Entrez Gene ID2625
RefSeq Protein Accession(s)NP_002042; XP_005252499; XP_005252500; NP_001002295
RefSeq mRNA Accession(s)XM_005252442; NM_002051; XM_005252443; NM_001002295
RefSeq Genomic Accession(s)NC_000010; NG_015859
UniProt ID(s)P23771
PharmGKB ID(s)PA28586
KEGG Gene ID(s)hsa:2625
Associated Diseases (KEGG IDs)Hypoparathyroidism, sensorineural deafness, and renal disease (HDR) [MIM:146255]: A disease characterized by steroid-resistant nephrosis with progressive renal failure, hypoparathyroidism, sensorineural deafness, and renal dysplasia. {ECO:0000269|PubMed:10935639, ECO:0000269|PubMed:11389161, ECO:0000269|PubMed:26514990}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of GATA3 .
GATA3 binding protein 3 or GATA3, is a zinc finger transcription factor and plays an important role in promoting and directing cell proliferation, development, and differentiation in many tissues and cell types. The human GATA3 gene has been mapped to chromosome 10p15. GATA =3 expression is primarily seen in breast carcinoma and urothelial carcinoma. Anti-GATA3 can also be useful in the identification of unknown primary carcinoma when carcinomas of the breast or bladder are a possibility

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment EDTA Buffer pH8.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-GATA3 Antibody Clone: L50-823
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Breast Carcinoma, Urothelial Carcinomas
Cellular Localization of Antibody L50-823 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Peptide between trans-activation and DNA-binding domains of GATA-3
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

GATA3 Information for Pathologists

Summary:

One of 6 members of the GATA family of transcription factors. Involved in the luminal differentiation of breast epithelium, development of collecting system / urothelium and trophoblastic differentiation. Also master regulator of type 2 helper T cells. Essential features Nuclear marker with expression in many epithelial neoplasms (including most breast, urothelial, paraganglioma / pheochromocytoma and skin carcinoma; smaller percentages of lung, liver, pancreatic, gastric, renal, thyroid, endometrial, ovarian and salivary gland carcinoma).

Notable Clinical Features:

GATA3 mutations cause HDR (hypoparathyroidism, sensorineural deafness and renal dysplasia) syndrome (Ann N Y Acad Sci 2011;1237:24, Endocr J 2011;58:117). Uses by pathologists Differentiate metastatic urothelial and breast carcinomas (GATA3+) from many other metastatic carcinomas (Am J Surg Pathol 2014;38:13). Differentiate urothelial carcinoma (> 80% GATA3+) from prostatic carcinoma (2% GATA3+). Differentiate metastatic lobular carcinoma of the breast (GATA3+) from gastric signet ring cell carcinoma (GATA3-).

Common Uses By Pathologists:

Differentiate metastatic urothelial and breast carcinomas (GATA3+) from many other metastatic carcinomas (Am J Surg Pathol 2014;38:13). Differentiate urothelial carcinoma (> 80% GATA3+) from prostatic carcinoma (2% GATA3+). Differentiate metastatic lobular carcinoma of the breast (GATA3+) from gastric signet ring cell carcinoma (GATA3-). Differentiate squamous cell carcinoma of the skin (GATA3+) from squamous cell carcinoma of the lung (GATA3-). Differentiate mesothelioma (81% GATA3+) from pulmonary adenocarcinoma (12% GATA3+).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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GATA3 [L50-823] Antibody (cGMP).