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Anti-GLG1 Antibody Clone GLG1/970

$ 298.00
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SKU: 2734-MSM1
Species: Human, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunocytochemistry, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 2734-MSM1-P1
GLG1 General Information
Alternate Names
Golgi apparatus protein 1, GLG1
Molecular Weight
134kDa
Chromosomal Location
16q22.3
Curated Database and Bioinformatic Data
Gene SymbolGLG1
Entrez Gene ID2734
Ensemble Gene IDENSG00000090863
RefSeq Protein Accession(s)NP_001139138, NP_036333, NP_001139139
RefSeq mRNA Accession(s)NM_001145666, NR_027264, NM_001145667, NM_012201, NR_027265
RefSeq Genomic Accession(s)NC_000016, NC_018927
UniProt ID(s)Q92896
UniGene ID(s)Q92896
HGNC ID(s)4316
Cosmic ID(s)GLG1
KEGG Gene ID(s)hsa:2734
PharmGKB ID(s)PA28719
General Description of GLG1.
This MAb recognizes a protein of 134kDa, which binds fibroblast growth factor, E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues, 16 Cys-rich GLG1 repeats. This MAb can be used to stain the Golgi complex in cell or tissue preparations, can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal, malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process, package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region, trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced, numerous in cells that make, secrete many substances such as plasma B cells.

Human, Mouse (-), and Rat (-) Anti-GLG1 Antibody Product Attributes

Species: Human, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunocytochemistry, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1.0ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1.0ug of antibody/ml), Immunocytochemistry (Acetone or paraformaldehyde fixed) (0.5-1ug of antibody/ml for 30 min), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-GLG1 Antibody Clone: GLG1/970
Clone GLG1/970 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse (-), and Rat (-) GLG1 Positive Control Sample: HepG2, A431 or HeLa cells. Placenta, Tonsil, Testis, Ovary.
Cellular Localization of Antibody Golgi complex in cytoplasm
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Golgi fraction from human liver cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

GLG1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-GLG1 antibody staining is expected to be primarily localized to the golgi apparatus.

Observed Antibody Staining Data By Tissue Type:

Variations in GLG1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the glomeruli in kidney, cells in the molecular layer in cerebellum, cells in the red pulp in spleen, cells in the tubules in kidney, decidual cells in the placenta, fibroblasts in skin and mesenchymal tissue, germinal center cells in the tonsil, glandular cells in the adrenal gland, appendix, colon, duodenum, endometrium, epididymis, gallbladder, prostate, salivary gland and stomach, islets of Langerhans in pancreas, Leydig cells in the testis, lymphoid tissue in appendix, macrophages in lung, neuronal cells in the caudate nucleus, cerebral cortex and hippocampus, neuropil in cerebral cortex, non-germinal center cells in the tonsil, peripheral nerve in mesenchymal tissue, Purkinje cells in the cerebellum, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. More moderate antibody staining intensity was present in cells in the glomeruli in kidney, cells in the molecular layer in cerebellum, cells in the red pulp in spleen, cells in the tubules in kidney, decidual cells in the placenta, fibroblasts in skin and mesenchymal tissue, germinal center cells in the tonsil, glandular cells in the adrenal gland, appendix, colon, duodenum, endometrium, epididymis, gallbladder, prostate, salivary gland and stomach, islets of Langerhans in pancreas, Leydig cells in the testis, lymphoid tissue in appendix, macrophages in lung, neuronal cells in the caudate nucleus, cerebral cortex and hippocampus, neuropil in cerebral cortex, non-germinal center cells in the tonsil, peripheral nerve in mesenchymal tissue, Purkinje cells in the cerebellum, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. Low, but measureable presence of GLG1 could be seen in cells in the seminiferous ducts in testis, epidermal cells in the skin, glial cells in the caudate nucleus, cerebral cortex and hippocampus and pneumocytes in lung. We were unable to detect GLG1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of GLG1 expression as measured by anti-GLG1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ ++ ++ +++ +++ ++ ++ +++ ++ + ++ ++ ++ ++ + + ++ +++ +++ +++
GLG1 Variability ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ + ++

Limitations and Warranty

enQuire Bio's GLG1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-GLG1 Antibody Clone GLG1/970