Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-Glial Fibrillary Acidic Protein Antibody Clone: GA5
Host and Isotype: Mouse IgG1, kappa
Recommended Positive Control Sample: Brain
Cellular Localization of Antibody GA5 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were immunized with glial fibrillary protein from porcine spinal cord.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Glial Fibrillary Acidic Protein Information for Pathologists
Summary:
Intermediate filament for astrocytes (normal, reactive, neoplastic). Positive staining - disease CNS tumors, colonic schwannoma (Am J Surg Pathol 2001;25:846). Microscopic (histologic) imagesCommon Uses By Pathologists:
CNS tumors and colonic schwannoma (Am J Surg Pathol 2001;25:846) as well as myxopapillary ependymoma of broad ligament stain positive for GFAP.Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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