Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with Glypican-3 Monoclonal Antibody (1G12)
Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with Glypican-3 Monoclonal Antibody (1G12)

Anti-Glypican-3 Antibody Clone 1G12

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 2719-MSM1
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
2719-MSM1-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
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SKU: 2719-MSM1-P1
Glypican-3 General Information
Alternate Names
Glypican-3, GPC3
Molecular Weight
67kDa
Chromosomal Location
Xq26.2
Curated Database and Bioinformatic Data
Gene SymbolGPC3
Entrez Gene ID2719
Ensemble Gene IDENSG00000147257
RefSeq Protein Accession(s)XP_016884902, NP_001158090, NP_001158089, NP_001158091, NP_004475
RefSeq mRNA Accession(s)XM_017029413, NM_001164618, NM_001164617, NM_004484 NM_001164619
RefSeq Genomic Accession(s)NC_018934, NG_009286, NC_000023
UniProt ID(s)Q53H15, B4DTD8, I6QTG3, P51654
UniGene ID(s)Q53H15, B4DTD8, I6QTG3, P51654
HGNC ID(s)4451
Cosmic ID(s)GPC3
KEGG Gene ID(s)hsa:2719
PharmGKB ID(s)PA28832
General Description of Glypican-3.
Recognizes a protein, which is identified as HGAL. It contains a putative PDZ-interacting domain, an immunoreceptor tyrosine-based activation motif (ITAM),, two putative SH2 binding sites. In B cells, its expression is specifically induced by interleukin-4. HGAL is specifically expressed in germinal center B-cells, but is absent in mantle, marginal zone B-cells, in the inter-follicular, para-cortical regions in normal tonsils, lymph nodes. Its high degree of specificity for germinal center B-cells makes anti-HGAL an ideal marker for the detection of germinal center-derived B-cell lymphomas. HGAL expression has been used to help elucidate nodal marginal zone lymphoma (NMZL) from cases of diffuse follicle center lymphoma. Additionally, HGAL expression was shown to correlate with survival in patients with diffuse large B-cell lymphoma (DLBCL).

Human Anti-Glypican-3 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Glypican-3 Antibody Clone: 1G12
Clone 1G12 Host and Isotype: Mouse IgG1 kappa
Anti-Human Glypican-3 Positive Control Sample: 293T cells or Hepatocellular carcinoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A recombinant fragment containing amino acids 511-580 of human glypican-3
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Glypican-3 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-GPC3 antibody staining is expected to be primarily localized to the plasma membrane.

Limitations and Warranty

enQuire Bio's Glypican-3 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Glypican-3 Antibody Clone 1G12

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