Formalin-fixed, paraffin-embedded human Melanoma stained with Glypican-3 Monoclonal Antibody (1G12 + GPC3/863)
Formalin-fixed, paraffin-embedded human Melanoma stained with Glypican-3 Monoclonal Antibody (1G12 + GPC3/863)

Anti-Glypican-3 Antibody Clone 1G12 + GPC3/863

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 2719-MSM3
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG
Mass Spec Validated?: Not MS Validated

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In Stock: 1-2 Day Lead Time.
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Datasheets and Documentation
Product Datasheet
SKU: 2719-MSM3-P1
Glypican-3 General Information
Alternate Names
Glypican-3, GPC3
Molecular Weight
67kDa
Chromosomal Location
Xq26.2
Curated Database and Bioinformatic Data
Gene SymbolGPC3
Entrez Gene ID2719
Ensemble Gene IDENSG00000147257
RefSeq Protein Accession(s)XP_016884902, NP_001158090, NP_001158089, NP_001158091, NP_004475
RefSeq mRNA Accession(s)XM_017029413, NM_001164618, NM_001164617, NM_004484 NM_001164619
RefSeq Genomic Accession(s)NC_018934, NG_009286, NC_000023
UniProt ID(s)Q53H15, B4DTD8, I6QTG3, P51654
UniGene ID(s)Q53H15, B4DTD8, I6QTG3, P51654
HGNC ID(s)4451
Cosmic ID(s)GPC3
KEGG Gene ID(s)hsa:2719
PharmGKB ID(s)PA28832
General Description of Glypican-3.
Glypican-3 (GPC3) is an integral membrane protein that is mutated in the Simpson-Golabi-Behmel syndrome (SGBS). SGBS is characterized by pre-, post-natal overgrowth, is a recessive X-linked condition.GPC3 may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors,, Wilm s tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue, elevated in serum, but is undetectable in normal liver, benign liver,, the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules, areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors, choriocarcinoma. A high level of GPC3 expression has also been found in some types of embryonal tumors, such as Wilm s tumor, hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma, 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma was significantly higher than that of follicular adenoma. In contrast, GPC 3 is not expressed in anaplastic carcinoma.

Human Anti-Glypican-3 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Glypican-3 Antibody Clone: 1G12 + GPC3/863
Clone 1G12 + GPC3/863 Host and Isotype: Mouse IgG
Anti-Human Glypican-3 Positive Control Sample: 293T cells or Hepatocellular carcinoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant fragment containing amino acids 511-580 of human glypican-3 (1G12); Recombinant full-length human GPC3 protein (GPC3/863)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Glypican-3 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-GPC3 antibody staining is expected to be primarily localized to the plasma membrane.

Limitations and Warranty

enQuire Bio's Glypican-3 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Glypican-3 Antibody Clone 1G12 + GPC3/863

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